甘薯质体中的乙酰辅酶A羧化酶亚基基因accD的克隆与序列分析  

Cloning and Sequence Analyses of Plastid-Encoded Acetyl-CoA Carboxylase Subunit Gene accD of Sweet Potato [Ipomoea batatas(L.) Lam.]

作　　者：王玉华[1] 郝建国[1] 贾敬芬[1] 

机构地区：[1]西北大学生命科学学院,陕西省生物技术重点实验室,西部资源生物与现代生物技术教育部重点实验室,西安710069

出　　处：《植物生理学通讯》2009年第11期1065-1069,共5页Plant Physiology Communications

基　　金：陕西省教育厅专项(09JK777);国家自然科学基金(30900914和30671082);西北大学西部资源生物与生物技术重点实验室开放基金

摘　　要：依据烟草质体全基因组序列设计引物,以甘薯质体基因组DNA为模板,PCR扩增包含质体accD基因完整编码区在内的一段序列(GenBank登录号为GQ395771)。序列分析表明:该片段全长为2209bp,包括1548bp的accD基因编码序列,推测编码515个氨基酸的蛋白质,该蛋白序列具有异质型β-CT中保守的锌指结构和C末端5个基元。同时绘制了该DNA片段的限制性酶切图谱。相似性比较显示,甘薯accD基因与大豆、马铃薯、拟南芥、人参、莴苣、葡萄、海岛棉、甘蓝、辣椒、菠菜、番茄和烟草的accD基因核苷酸相似性为72%～87%,氨基酸相似性为58%～83%。The fragment containing plastid accD gene （GenBank accession number： GQ395771） encoding the carboxyltransferase β subunit of acetyl-CoA carboxylase （ACCase） was cloned from plastid genome DNA in sweet potato （Ipomoea batatas）. Sequencing analysis indicated that sweet potato accD is 2 209 bp with an 1 548 bp open reading frame （ORF）, encoding a 515-amino-acid polypeptide which contained the zinc finger structure and the C-terminal regions with five motifs. The restriction map of this cloned fragment was also established. The BLAST results showed that the homologies of this gene with soybean, potato, tobacco, Arabidopsis thaliana, ginseng, lettuce, grape, sea-island cotton, cabbage, pepper, spinach, tomato and tobacco were from 72% to 87%, and the similarities of the amino acid sequences were from 58% to 83%.

关 键 词：甘薯 乙酰辅酶A羧化酶(ACCase) accD基因 克隆 

分 类 号：Q943.2[生物学—植物学] S531[农业科学—作物学]