新疆野生种与栽培种郁金香的杂交种子无菌萌发和鳞茎形成  

Aseptic Germination and Bulblet Formation of Interspecific Hybrids between Wild Species and Cultivar of Tulip(Tulipa L.)

作　　者：王彩霞[1] 欧阳彤[1] 姜彦成[2] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《植物生理学通讯》2009年第11期1098-1100,共3页Plant Physiology Communications

基　　金：国家自然科学基金(30560016)

摘　　要：新疆野生种与栽培种郁金香的杂交F1种子于4℃低温下培养36～67d后萌发,随后于15℃下培养3～5个月形成鳞茎。‘小黑人’×柔毛郁金香、‘小黑人’×天山郁金香和‘小黑人’×伊犁郁金香F1种子的萌发率差异显著,分别为60.37%～63.89%、80.28%～81.25%和3.75%～4.45%。‘小黑人’×柔毛郁金香和‘小黑人’×天山郁金香F1的鳞茎分别在添加6-BA2.0mg·L-1(单位下同)+KT1.0+TDZ0.1和6-BA5.0+KT0.4的1/2MS培养基中增殖。‘小黑人’×天山郁金香F1种子萌发后有41.67%呈畸形,畸形的萌芽在添加6-BA2.0+KT0.4的1/2MS培养基中可诱导出鳞茎并增殖。The F1 seeds of interspecific hybrids between wild species and cultivar of tulip （Tulipa L.） germinated after 36-67 days cultured in 4℃ and formed subsequently bulblets after 3-5 months cultured in 15℃. The germination rates of F1 seeds of ＇Negrita＇× Tulipa buhseana, ＇Negrita＇×T. tianschanica, and ＇Negrita＇×T. iliensis were 60.37%-63.89%, 80.28%-81.25%, 3.75%--4.45% respectively and there were significant differences among them. ＇Negrita＇×T. buhseana F1 bulblets proliferated in 1/2MS medium supplemented with 2.0 mg.L^-1 6-BA, 1.0 mg.L^-1 KT and 0.1 mg.L^-1 TDZ, meanwhile, ＇Negrita＇×T. tianschanica F1 bulblets proliferated in 1/2 MS medium supplemented with 5.0 mg.L^-1 6-BA and 0.4 mg.L^-1 KT. There were 41.67% ＇Negrita＇×T. tianschanica showed abnormal morphology, which could induce bulblets in 1/2MS medium supplemented with 2.0 mg.L^-1 6-BA and 0.4 mg.L^-1 KT.

关 键 词：郁金香 种间杂种 种子萌发 鳞茎形成 

分 类 号：S682.263[农业科学—观赏园艺] S633.4[农业科学—园艺学]