补充辅酶Q_(10)对大鼠血清总SOD活力及MDA含量的影响  

Effects of exogenous CoQ_(10) on SOD activity and MDA content in serum of rats

作　　者：李洁[1] 张军[1] 王玉侠[2] 王茂生[3] 师德明[1] 

机构地区：[1]西北师范大学体育学院,甘肃兰州730070 [2]上海体育学院,上海200438 [3]甘肃省委党校,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2009年第6期98-101,105,共5页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省自然科学基金资助项目(ZGS064-A43-020-05)

摘　　要：以运动、外源性补充CoQ10以及运动合并补充CoQ10为干预手段,观察大鼠血清总超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的变化,探讨在运动训练中补充CoQ10的抗氧化作用.选36只健康雄性Wister大鼠,随机分为4组,即安静对照组(NC)、安静状态补充CoQ10组(QC)、运动训练组(NE)和运动训练状态补充CoQ10组(QE),灌胃补充CoQ10及递增负荷运动训练7周,力竭运动后即刻断头取血,分光光度法测定血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力.结果表明:①与NC组比较,QC组血清MDA含量增多(P<0.01),总SOD活力无显著性差异;NE组血清MDA含量下降,总SOD活力提高(P<0.01);QE组血清MDA含量减少,总SOD活力提高(P<0.01).②与QC组相比,QE组血清MDA含量下降,总SOD活力提高(P<0.01).③与NE组相比,QE组血清MDA含量及总SOD活力无显著性差异.To examine the effects of exogenous coenzyme Q10（CoQ10） on the activity of SOD and cotent of MDA in serum of rats and explore the function of CoQ10 on anti-oxidation,36 healthy male Wister rats are randomly divided into 4 groups（n=9） as static control（NC）,supplementation of CoQ10（QC）,exercise-training（NE） and training with supplementation of CoQ10（QE）.Filling the stomach to supplement CoQ10 and the treadmill training for 7 weeks,beheads draws blood o-hour single bout of exhausted exercise.The SOD activities and MDA content are mensurated by spectrophotometric in serum. Compared with NC, QC lead to a significant increase of MDA content in serum（P〈0.01）, but no effect on the activity of total SOD; NE lead to a significant decline of MDA contents（P〈0.01）and a significant increase of total SOD activities in serum（P〈0. 01）; QE result in a significant increase of total SOD activities（P〈0.01）and a significant decline of MDA contents in serum（P〈0.01）. Compared with QC, OE lead to a significant decline in MDA content（P〈0.01）and a marked increase of SOD activities（P〈0.01）in serum. Compared with NE, QE could not effect MDA contents and SOD activities in serum.

关 键 词：运动训练 辅酶Q10 血清 超氧化物歧化酶 丙二醛 大鼠 

分 类 号：Q552[生物学—生物化学]