种子特异启动子的GPD1基因植物表达载体构建  

Construction of A Seed-specific Plant Expression Vector Targeting the GPD1 Gene

作　　者：李群[1,2] 王学英[1] 刘野[2,3] 阮成江[2] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161 [2]大连民族学院生命科学院,辽宁大连116600 [3]辽宁师范大学,辽宁大连116029

出　　处：《华北农学报》2009年第5期31-34,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家"十一五"科技支撑计划重大项目子专题(2006BAD09A04);中国博士后科学基金特别资助(200801371);中国博士后科学基金(20070420990);江苏省博士后基金(0702018C)

摘　　要：利用PCR技术从甘蓝型油菜(Brassica napus L.)和酵母INVSc1(Saccharomyces cerevisiae)的基因组DNA中分别扩增获得种子特异启动子napin和3-磷酸甘油脱氢酶(GPD1)基因片段,并将它们分别克隆到pMD18-T载体中。利用中间载体pBluescriptKS将两目的片段插入到植物表达载体pCAMBIA1390的多克隆位点处,构建了种子特异启动子napin驱动的GPD1基因植物表达载体p1390NG,并用冻融法将其导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404、EHA105和AGL1中,可应用于油料能源植物种子含油量的遗传改良。By the PCR technique,seed-specific promoter（napin） and glycerol-3-phosphate dehydrogenase（GPD1）were amplified from Brassica napus and Saccharomyces cerevisiae INVSc1 genomic DNA,and cloned into the pMD18-T vector,respectively.The two fragments were inserted into the multiple cloning sites of the binary vector pCAMBIA1390 using the pBluescriptKS as the intermediary cloning vector.The resulted seed-specific expression vector was named as p1390NG.The vector was transformed into the Agrobacterium tumefaciens strains LBA4404, EHA105 and AGL1. It may provide an excellent genetic resources for genetic improvement of the seed oil content of energy plant.

关 键 词：甘蓝型油菜 酵母INVSc1 NAPIN 3-磷酸甘油脱氢酶(GPD1) 基因表达载体 

分 类 号：Q785[生物学—分子生物学]