木质素降解菌的筛选及对秸秆的降解研究  

Study on the Screening of the Lignin-degrading Fungus and the Degradation of Straws

作　　者：梁军锋[1] 张洪生[1] 张克强[1] 张金凤[1] 李野[1] 赵润[1] 

机构地区：[1]农业部环境保护科研监测所资源再生研究室,天津300191

出　　处：《华北农学报》2009年第5期206-209,共4页Acta Agriculturae Boreali-Sinica

基　　金：农业部农业结构调整重大技术研究专项(D-07-02B);中央公益性科研院所基本科研业务费专项(2009-DLI-04)

摘　　要：为筛选木质素高效降解菌,对筛选的方法体系进行了研究。结果表明,利用三种定性筛选平板结合定量的木质素磺酸钙降解的方法,能明显提高筛选效率,从16个菌株中筛选到3株对木质素有较强降解能力的菌株Tp1、Tf1与Ls21。为进一步验证所筛菌株的降解效果,选取其中综合性能较好的Tf1菌株(凤尾菇,Pleurotus sajor-caju),对小麦秸秆与玉米秸秆进行固态发酵试验,并以黄孢原毛平革菌(Phanerochaete chrysosporium)BKMF-1767作参照,结果表明,Tf1对小麦秸秆与玉米秸秆木质素的降解率分别为38.4%与47.7%,均高于参照菌株(分别为32.2%和34.6%)。To use three kinds of screening plate,and to combine with quantitative degradation test of calcium lignosulphonate,three lignin-degradation strains,TP1,Tf1 and Ls21 were obtained.In order to confirm the degradation effect of the strains,the strain Tf1（Pleurotus sajor-caju）,was selected to do solid substrate fermentation test about the wheat straw and corn straw.And Phanerochaete chrysosporium（BKMF-1767） was made as a reference.The result showed that the degradation rate of Tf1 to the wheat straw and corn was 38.4% and 47.7% respectively,and it was higher than the reference strain（32.2% and 34.6% ）. This research improves the screening method system of lignin-degrading fungus. There is an important significance to expand the resource of lignin-degrading fungus and exploit the straw resource.

关 键 词：秸秆 木质素 筛选 降解 白腐菌 

分 类 号：S182[农业科学—农业基础科学]