葡聚六糖诱导黄瓜结构抗性及系统信号传导机制  

Mechanisms of System Signal Transduction and Constriction Resistance in Cucumber Induced by Glucohexaose

作　　者：孙艳秋[1] 李宝聚[2] 石延霞[2] 陈捷[3] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]上海交通大学农业与生物学院,上海201101

出　　处：《吉林农业大学学报》2009年第4期376-381,共6页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30270910);北京市自然科学基金重点项目(6021004)

摘　　要：为研究葡聚六糖诱导黄瓜对霜霉病的系统抗性机制,采用生理生化方法测定了诱导后黄瓜不同叶位叶片中木质素和可溶性酚含量,苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)活性,Ca2+、水杨酸(SA)和茉莉酸(JA)的含量变化。结果表明:葡聚六糖可以诱导黄瓜对霜霉病的系统抗性,经葡聚六糖处理的植株不同叶位叶片木质素和可溶性酚含量,PAL和POD活性,Ca2+、SA和JA含量都明显升高,说明葡聚六糖是通过积累结构抗性物质,经SA和JA传递抗病信号,从而诱导黄瓜对霜霉病的系统抗性。In order to explore the mechanism of the systemic resistance to cucumber downy mildew induced by plant glucohexaose, the physiological and biochemical method was employed to study the changes in contents of lignin, phenolic compound, Ca^2 ＋ , Salicylic acid （SA）, Jasmonic Acid （JA） and activities of Phenylalanine ammonia-lyase（PAL）, Peroxidase （POD） in cucumber leaves at different positions pretreated with glucohexaose. The results showed that glucohexaose could induce system resistance of cucumber treated leaves, upper leaves from the treated leaves, lower leaves from the treated leaves to Pseudoperonospora cubensis, and the contents of lignin, phenolic compound, Ca^2＋ , SA and JA, activities of PAL and PPO increased in the leaves at different positions treated with glucohexaose. This indicated that glucohexaose could induce system resistance of cucumber to Pseudoperonospora cubensis by accumulating constriction resistance materials, by way of SA and JA transferring disease resistance signal.

关 键 词：葡聚六糖 黄瓜 结构抗性 系统信号传导 

分 类 号：S482.8[农业科学—农药学]