鹿源牛病毒性腹泻—黏膜病病毒E_2基因的克隆与序列分析  

Cloning and Sequence Analysis of E_2 Gene of Bovine Viral Diarrhea Virus Isolated from Deer

作　　者：杜锐[1] 刘苓钰[1] 徐兴然[2] 时坤[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]西南大学动物科技学院,重庆400716

出　　处：《吉林农业大学学报》2009年第4期443-446,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30671572);吉林省杰出青年基金项目;省部共建教育部重点实验室资助项目

摘　　要：对已发表的牛病毒性腹泻—黏膜病毒E2基因的核苷酸序列进行比较分析并设计引物,应用套式RT-PCR获得鹿源BVDV E2基因。测序分析结果表明:鹿源BVDV E2基因由1 122个核苷酸组成,编码374个氨基酸。与Oregon CV24、NADL、ZM-95、SD1、Osloss、S10、SH9、Changchun184、Shitara、Deer-GB1核苷酸和推导氨基酸同源性分析表明:鹿源DVBV分离株与Changchun 184株及Osloss株的同源性较高,分别为99.2%和92.8%;与日本Shitara株的同源性为71.2%;与国际标准毒株CV24的同源性为74.6%。The major antigenic coding gene E2 of deer isolated of Bovine Viral Diarrhea Virus was obtained by using reverse transcription polymerase chain reaction （RT- PCR） and nest-PCR. Sequencing result showed that the E2 gene of isolated strain has 1 122 nucleotides in length, which encode amino acid 374 .The homology analysis of the nucleotide and amino acid of the sequences about E2 gene indicates that the isolated strain has the best homology with Changchun184 strain 99.2% and the minimum with Shinara 71.2%. The others are Osloss 92.8%, CV24 74.6%, SD1 74.6%, NADL 73.5%, SH9 72.7%,ZM-95 72.5% and Deer-GB1 73.0%,S10 72.1%.

关 键 词：鹿 牛病毒性腹泻—黏膜病病毒 E2基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学] S852.65[农业科学—基础兽医学]