抗生素对2个越橘品种叶片再生的影响  

Effects of Antibiotics on Regeneration of Leaves of Two Blueberry Cultivars

作　　者：邢瑞丹[1] 刘庆忠[2] 陈新[2] 李亚东[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]山东省果树研究所山东省果树生物技术重点实验室,泰安271000

出　　处：《吉林农业大学学报》2009年第5期524-527,共4页Journal of Jilin Agricultural University

基　　金：公益性行业(农业)科研专项基金项目(nyhyzx07-028;2006-G25);吉林省科技发展重点项目(2007-5013);山东省农业科学院自主创新基金项目(2007YCX010)

摘　　要：以Sunrise和Geo组培苗幼叶为试材,研究了卡那霉素、头孢霉素和羧苄青霉素对越橘叶片再生不定芽的影响。用继代约20d的新梢顶部新展开叶片制备外植体,Sunrise接种在WPM+ZT4.0mg/L+TDZ1.0mg/L+IBA0.3mg/L+蔗糖20.0g/L+琼脂6.0g/L的再生培养基上,Geo接种在WPM+ZT4.0mg/L+IBA0.3mg/L+蔗糖20.0g/L+琼脂6.0g/L的再生培养基上,添加不同浓度的抗生素。结果表明:10mg/L卡那霉素能极显著地抑制越橘叶片再生,600mg/L头孢霉素能完全抑制叶片再生,500mg/L羧苄青霉素能完全抑制叶片再生。Young leaves of Sunrise and Geo were selected as experimental materials. Effects of kanamycin, cefotaxime and carbenieillin on regeneration from leaves in vitro were studied. Leaves on the top of shoots of Sunrise were cultured on WPM medium containing ZT 4.0 mg/L, TDZ 1.0 mg/L, IBA 0.3 mg/L, sucrose 20.0 g/L, agar 6.0 g/L, Geo were cultured on WPM medium containing ZT 4.0 mg/L, IBA 0.3 mg/L, sucrose 20.0 g/L, agar 6.0 g/L, and several kinds of doses of antibiotics respectively. Results showed that Kanamycin strongly inhibited regeneration even at a dose of 10 mg/L, and at a dose of 20 mg/L adventitious buds could not grow as usual and died, cefotaxime at a dose of 600 mg/L and while carbenicillin at a dose of 500 mg/L inhibited regeneration.

关 键 词：越橘 离体叶片 再生 抗生素 

分 类 号：S663.9[农业科学—果树学]