Evolutionary Implication of Outer Membrane Lipoprotein-Encoding Genes ompL1,lipL32 and lipL41 of Pathogenic Leptospira Species  

作　　者：K. Vedhagiri K. Natarajaseenivasan P. Chellapandi S.G. Prabhakaran Joseph Selvin S. Sharma P. Vijayachari 

机构地区：[1]Division of Medical Microbiology,Department of Microbiology,School of Life Sciences, Bharathidasan University,Tiruchirappalli 620024,Tamilnadu,India [2]Department of Bioinformatics,School of Life Sciences,Bharathidasan University,Tiruchirappalli 620024,Tamilnadu,India [3]Regional Medical Research Centre(RMRC),Indian Council of Medical Research/WHO Collaborative Centre for Diagnosis,Research,Reference and Training in Leptospirosis,Port Blair 744101,Andaman and Nicobar Islands,India

出　　处：《Genomics, Proteomics & Bioinformatics》2009年第3期96-106,共11页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by grants from the Department of Science and Technology,Government of India (Sanction order No. SR/FT/L-47/2006)

摘　　要：Leptospirosis is recognized as the most widespread zoonosis with a global distribution. In this study, the antigenic variation in Leptospira interrogans and Leptospira borgpetersenii isolated from human urine and field rat kidney was preliminarily confirmed by microscopic agglutination test using monoclonal antibodies, and was further subjected to amplification and identification of outer membrane lipoproreins with structural gene variation. Sequence similarity analysis revealed that these protein sequences, namely OmpL1, LipL32 and LipL41, showed no more homologies to outer membrane lipoproteins of non-pathogenic Leptospira and other closely related Spirochetes, but showed a strong identity within L. interrogans, suggesting intra-specific phylogenetic lineages that might be originated from a common pathogenic leptospiral origin. Moreover, the ompL1 gene showed more antigenic variation than lipL32 and lipL41 due to less conservation in secondary structural evolution within closely related species. Phylogenetically, ompL1 and lipL4l of these strains gave a considerable proximity to L. weilii and L. santaro- sai. The ompI,1 gene of L. interrogans clustered distinctly from other pathogenic and non-pathogenic leptospiral species. The diversity of ompL genes has been an- alyzed and it envisaged that sequence-specific variations at antigenic determinant sites would result in slow evolutionary changes along with new serovar origination within closely related species. Thus, a crucial work on effective recombinant vaccine development and engineered antibodies will hopefully meet to solve the therapeutic challenges.Leptospirosis is recognized as the most widespread zoonosis with a global distribution. In this study, the antigenic variation in Leptospira interrogans and Leptospira borgpetersenii isolated from human urine and field rat kidney was preliminarily confirmed by microscopic agglutination test using monoclonal antibodies, and was further subjected to amplification and identification of outer membrane lipoproreins with structural gene variation. Sequence similarity analysis revealed that these protein sequences, namely OmpL1, LipL32 and LipL41, showed no more homologies to outer membrane lipoproteins of non-pathogenic Leptospira and other closely related Spirochetes, but showed a strong identity within L. interrogans, suggesting intra-specific phylogenetic lineages that might be originated from a common pathogenic leptospiral origin. Moreover, the ompL1 gene showed more antigenic variation than lipL32 and lipL41 due to less conservation in secondary structural evolution within closely related species. Phylogenetically, ompL1 and lipL4l of these strains gave a considerable proximity to L. weilii and L. santaro- sai. The ompI,1 gene of L. interrogans clustered distinctly from other pathogenic and non-pathogenic leptospiral species. The diversity of ompL genes has been an- alyzed and it envisaged that sequence-specific variations at antigenic determinant sites would result in slow evolutionary changes along with new serovar origination within closely related species. Thus, a crucial work on effective recombinant vaccine development and engineered antibodies will hopefully meet to solve the therapeutic challenges.

关 键 词：Leptospiru OMPL1 LIPL32 lipL41 PHYLOGENY antigenic variation 

分 类 号：R514.4[医药卫生—内科学]