胆红素对酶法和碱性苦味酸法测定肌酐的影响  

The effect of bilirubin on measuring creatinine by enzymatic method and alkaline picrate method

作　　者：王卉妍[1] 

机构地区：[1]解放军316医院检验科,北京100093

出　　处：《医疗装备》2009年第12期39-40,共2页Medical Equipment

摘　　要：目的:应用酶法和碱性苦味酸法测定消除胆红素前后的血清肌酐,探讨胆红素对这碱性苦味酸肌酐测定方法的影响程度。方法:收集临床黄疸血清标本30份,用碱性苦味酸法和酶法的方法测定肌酐水平,经铁氰化钾消除胆红素后再用碱性苦味酸方法再行测定。将处理前后的肌酐测定结果进行t检验,观察处理前后肌酐测定值有无差异。结果:30份临床黄疸标本分别用碱性苦味酸法(方法Ⅰ)酶法(方法Ⅱ)以及用铁氰化钾消除胆红素影响后的碱性苦味酸法(方法Ⅲ)测定肌酐值,结果发现方法Ⅱ和方法Ⅰ及方法Ⅲ之间差别有显著意义(p<0.01),胆红素对肌酐的碱性苦味酸法的测定有显著干扰,而对酶法测得的肌酐测定干扰不明显。结论:发现胆红素影响碱性苦味酸法测定肌酐,而不影响酶法对测定肌酐。Objective The purpose of this study was to assess whether bilirubin interfere the measurement of serum creatinine with an enzymatic assay and a kinetic Jaffe reaction,named ＂Jaffe compensated＂.Method：T：A total of 30 samples were detected with the two methods and repeat measurement after deposal of bilirubin.The results from each method in two conditions was compared in statistics.Results：The serum creatinine concentration determine by ＂Jaffe compensated＂ differs from that before deposal of bilirubin,but not when it came to the enzymafic assay. （P 〈 0.01） . Conchusion： Bilirubin can decreases the results of serum creatinine concentration measured by ＂Jaffe compensated＂, but it has no effect on enzymatic assay method.

关 键 词：胆红素 T检验 肌酐测定 

分 类 号：R446[医药卫生—诊断学]