HPLC法测定利咽解毒颗粒中大黄素的含量  

Quantitative Determination of Emodin in Liyan Jiedu Keli by HPLC

作　　者：张烨[1] 周浓[1] 张海珠[1] 

机构地区：[1]大理学院药学院,云南大理671000

出　　处：《亚太传统医药》2009年第11期40-41,共2页Asia-Pacific Traditional Medicine

摘　　要：目的:建立测定利咽解毒颗粒中大黄素含量的HPLC方法。方法:色谱柱为Agilent zorbax SB-C18柱(4.6mm×150mm,5μm),流动相为甲醇(A)—0.4%磷酸(B)线性梯度洗脱(0～10min,55%A→65%A;10～15min,65%A→72%A;15～20min,72%A;20～25min,72%A→80%A;25～30min,80%A→85%A;30～35min,85%A→55%A),流速为1.0mL.min-1,检测波长为289nm。结果:大黄素在0.5～100μg.mL-1范围内,峰面积与质量浓度呈良好的线性关系(r=0.9992),平均回收率为100.43%,RSD为1.28%(n=9)。结论:本方法快速、简便、准确,可用于本制剂的质量控制。Objective：To establish a HPLC method for the quantitative determination of emodin in Liyan Jiedu Keli.Methods：HPLC was performed on Agilent zorbax SB-C18 （4.6mm×150mm,5μm）with column at room temperature.The mobile phase consisted of methanol （ A ）-0.4%water-phosphoric acid （ B ） was optimized with gradient elution as follows ： 0-10min, 55%A-65%A; 10-15 min, 65%A-72%A ; 15 -20min ,72% A ; 20 -25rain, 72% A -80% A ; 25-30rain, 80% A -85% A ;30 -35min, 85% A →55% A.The flow rate and detection wavelength were set at 1.0mL·min^-1 and 289 nm.Results：The linear range of emodin was 0.5-100μg-mL^-1 （r=0.9992）, the mean recovery was100.43% with RSD 1.28% （n=9）.Conclusion：The method is fast,simple and accurate,and it can be applied for the quality control of Liyan Jiedu Keli.

关 键 词：利咽解毒颗粒 HPLC 大黄素 含量测定 

分 类 号：R284[医药卫生—中药学]