除草剂丙酯草醚对油菜和牛繁缕乙酰乳酸合成酶活性的影响(英文)  

Effects of novel herbicide ZJ0273 on acetolactate synthase activity in oilseed rape and crickweed

作　　者：赵丹丹[1] 刘永立[1] 兰大伟[2] 韩爱良[1] 叶庆富[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310029 [2]浙江工商大学统计与数学学院,浙江杭州310018

出　　处：《浙江大学学报（农业与生命科学版）》2009年第6期633-638,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Supported by Key Program of National Natural Science Foundation of China(20632070);Knowledge Innovation Program of Chinese Academy of Sciences(KGCX3-SYW-203-3)

摘　　要：以组织培养的牛繁缕、油菜为试验材料,研究除草剂丙酯草醚对乙酰乳酸合成酶(ALS)活性的影响,探讨在施用丙酯草醚后,添加不同支链氨基酸对牛繁缕生长恢复的作用.结果表明,丙酯草醚对牛繁缕ALS活性有较大的抑制作用,对油菜ALS活性的抑制作用较小,这表明丙酯草醚对牛繁缕和油菜有较好的选择性.单独添加异亮氨酸(Ile)、亮氨酸(Leu)、缬氨酸(Val)可缓解丙酯草醚对牛繁缕生长的抑制作用;同时添加任意2种支链氨基酸(Ile、Leu、Val)可以增强对牛繁缕的生长恢复作用;当Ile、Leu、Val均以10mg?L-1同时添加时,可以完全抵消丙酯草醚对牛繁缕生长的抑制作用.据此推测,丙酯草醚可以抑制杂草体内支链氨基酸合成所需的ALS活性,因而可能属于ALS抑制剂.ZJ0273, propyl 4-[2-（4, 6-dimethoxy-2-pyrimidinyloxy） benzylamino] benzoate, is a novel herbicide used mainly in the oilseed rape field. The effects of ZJ0273 on acetolactate synthase （ALS） activity in the tissue-cultured oilseed rape and crickweed were studied under in vitro and in vivo conditions. The alleviating effects of branched chain amino acids （BCAAs） on the ZJ0273-induced growth inhibition of crickweed were also investigated. The results showed that ZJ0273 inhibited significantly the AIrS activity of crickweed, while no significant inhibition was found on that of oilseed rape. The ZJ0273- induced growth inhibition of crickweed could be alleviated by adding isoleucine （Ile）, leucine （Leu） or valine （Val）, respectively. The alleviating effect by the combinations of either two BCAAs was more significant than by single BCAA. When Ile, Leu and Val （10 mg· L-1 for each） were simultaneously added to the medium, the inhibition of crickweed were completely reversed. These results suggested that ZJ0273 might belong to the inhibitor of ALS.

关 键 词：除草剂 丙酯草醚 油菜 牛繁缕 乙酰乳酸合成酶 

分 类 号：S482.47[农业科学—农药学]