苦瓜对慢性肝损伤大鼠肝组织丙二醛及一氧化氮含量的影响  

Effects of bittermelon on the content of malondialdehyde and nitric oxide in chronic liver damage rats

作　　者：任明[1] 宋志宏[2] 陈瑞莞[3] 鞠金秀[3] 赵铭鹏[4] 

机构地区：[1]中山大学附属第一医院消化内科,510080 [2]中山大学中山医学院生化教研室,510089 [3]中山大学中山医学院 [4]中山大学

出　　处：《新医学》2009年第12期781-783,F0003,共4页Journal of New Medicine

基　　金：广东省科技计划资助项目(2009B030801163);国家大学生创新性实验计划资助项目[教高函(2007)15号文;中大教务(2008)4号];中山大学医科学生业余科研资助项目[医教2008(46)号;医教(2006)69号]

摘　　要：目的:观察苦瓜对四氯化碳慢性肝损伤大鼠肝组织丙二醛及一氧化氮(nitric oxide,NO)含量的影响,探讨苦瓜抗慢性肝损伤的作用机制。方法:将32只健康Wistar雄性大鼠随机分为低剂量苦瓜组及高剂量苦瓜组(饲料含苦瓜分别为10%和20%)、模型组、对照组,每组各8只,予相应饲料喂食。饲养过程中,除对照组外,其余3组大鼠均用四氯化碳制备慢性肝损伤动物模型。在752型分光光度计上用分光光度法测定各组动物肝组织丙二醛及一氧化氮含量,通过苏木素-伊红(HE)染色观察肝组织的病理学变化。结果:与模型组比较,低剂量组及高剂量组的肝细胞水肿、脂肪变性及纤维化等病理学损伤程度较轻;且其肝组织中的丙二醛和一氧化氮含量均明显降低,以高剂量组更明显。结论:苦瓜可降低慢性肝损伤大鼠肝组织的丙二醛和一氧化氮含量。同时,可减轻慢性肝损伤所致的纤维化等病理改变,其作用机制可能与其抗氧化作用有关。Objective： To observe the effect of bittermelon on the content of malondialdehyde （MDA） and nitric oxide （NO） in rats liver damage induced by carbon tetrachloride, and explore the mechanism of bittermelon against chronic liver injury. Methods： Thirty-two male Wistar rats were randomly divided into control group（ vehicle-treated group）, carbon tetrachloride-induced group （model group）, and carbon tetrachloride-induced group with bittermelon treatment including low dose and high dose（ feed containing bittermelon were 10% and 20% , lowdose group, high-dose group） , and eight rats were studied in each group. The contents of MDA and NO of animal liver tissue were measured by a 752-type spectrophotometer, respectively. The pathological changes in liver tissue were evaluated by hematoxylin-eosin（HE） staining. Results： Compared with model group, the liver injury of induced by carbon tetrachloride, including edema, fatty degeneration and fibrosis in hepatic cells, were significantly ameliorated by bittermelon-treatment including low-dose group and high-dose group. The contents of MDA and NO lniver tissue were markedly reduced, especially in high-dose group. Conclusion： The bittermelon can reduce the contents of MDA and NO in rats with chronic liver injury, and can downregulate the live damage including fibrosis. The mechanism may be related to antioxidant effects.

关 键 词：苦瓜 四氯化碳 慢性肝损伤 丙二醛 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]