胆固醇改性5-FU脂质体的制备及透瘢痕实验  

Preparation of Modified 5-FU Liposomes with Cholesterol and Its Penetration Study in Hypertrophic Scar Penetration

作　　者：毛小慧[1] 沃雁[1] 章一新[1] 贺蓉[2] 崔大祥[2] 

机构地区：[1]上海交通大学附属第九人民医院整形外科,上海200011 [2]上海交通大学微纳科学技术研究院,上海200240

出　　处：《上海交通大学学报》2009年第11期1808-1812,共5页Journal of Shanghai Jiaotong University

基　　金：国家高技术研究发展计划(863)项目(2007AA022004);上海市教委基金资助项目(05BZ21);上海市科委纳米技术专项(0852nm03000);上海市科委自然科学基金(08ZR1412600)

摘　　要：为了解脂质体抗瘢痕药物在增生性瘢痕中的透皮作用,应用胆固醇对5-FU脂质体进行改性处理.应用挤出仪控制粒径的大小后,观察粒径对包封率的影响,并进行体外透瘢痕实验,观察粒径对于渗透深度的影响作用.结果表明:脂质体成均一的大单层球形结构,包封率随粒径的变小而增大.粒径越小,透瘢痕能力越强.本实验证明经胆固醇改性之后的小粒径脂质体是一种较好的抗瘢痕药物载体,它的透瘢痕机制主要是变形作用和融合作用.To investigate liposomes anti-scarring drug＇s ability in hypertrophic scar penetration, the 5-FU liposomes were modified with cholesterol. After the sizes of liposomes were controlled by extrusion equipment, the encapsulation efficiency of 5-FU in the liposomes was then investigated and observed. In vitro experiments of the liposomes to penetrate into human skin and scar were performed to find out whether the sizes of the liposomes would affect the depth of scar-penetration. The results show that with the decrease of the vesicle size, the encapsulation efficiency of 5-Fu increases gradually. The smaller the size of liposomes is, the stronger its permeating ability would be. The experiment proves that the modified smallsized liposomes are fine anti-scar drug carrier which can enhance their ability to penetrate into scar by the deformation and integration mechanisms.

关 键 词：粒径 5-氟尿嘧啶 脂质体 胆固醇 透瘢痕 

分 类 号：R619.6[医药卫生—外科学]