血小板源性生长因子-BB在肺癌患者中的表达及意义  

Expression of platelet-derived growth factor-BB in patients with lung cancer

作　　者：桂嵘[1] 黄竹英[2] 刘竞[1] 李红卫[2] 聂新民[2] 

机构地区：[1]中南大学湘雅三医院输血科,湖南长沙410013 [2]中南大学湘雅三医院检验科

出　　处：《中国输血杂志》2009年第10期810-812,共3页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助课题(编号:30300383);湖南省自然科学基金资助课题(编号:07JJ3036)

摘　　要：目的探讨肺癌患者血清及外周血单个核细胞中血小板源性生长因子-BB(PDGF-BB)的表达水平及其意义。方法采用酶联免疫吸附试验(ELISA)检测30名肺癌患者和20名正常对照者外周血血清中PDGF-BB的水平,用半定量逆转录-聚合酶链反应(RT-PCR)方法检测受试者外周血单个核细胞(PBMCs)中PDGF-B链mRNA的表达水平。结果肺癌患者和正常对照者血清PDGF-BB水平分别为(389.64±36.42)、(28.36±8.35)pg/ml,外周血单个核细胞PDGF-B链mRNA的表达水平分别为(0.9287±0.0942)、(0.2275±0.0384)灰度比值,比较差异有统计学的意义(P<0.01)。结论检测血清PDGF-BB及PBMCs中PDGF-B链mRNA表达水平可能对肺癌诊断具有一定临床指导意义。Objective To investigate the expression levels of platelet-derived growth factor-BB （PDGF-BB） and its role in the pathogenesis of the patients with lung cancer. Methods The levels of serum PDGF-BB in 30 patients with lung cancer and 20 normal controls were measured by enzyme linked immunosorbent assay （ ELISA ）. The levels of PDGF-B mRNA in peripheral blood mononuclear ceils were measured by reverse transcription polymerase chain reaction （ RT-PCR）. Results The serum levels of PDGF-BB and the levels of PDGF-B mRNA in peripheral blood mononuclear ceils were significantly higher in patients with lung cancer, which were （389. 64 ± 36. 42 ）pg/ml and （0. 928 7 ± 0. 094 2 ）, respectively, vs. （28. 36 ± 8.35 ） pg/ml and（0. 227 5 ± 0. 038 4） in normal controls （ P 〈 0.01 ）. Conclusions PDGF-BB may play a role in the pathogenesis of lung cancer. Testing the levels of PDGF-BB, PDGF-B mRNA may help with the diagnosis of lung cancer.

关 键 词：血小板源性生长因子-BB(PDGF-BB) 肺癌 酶联免疫吸附试验(ELISA) 逆转录-聚合酶链反应(RT-PCR) 

分 类 号：R446.6[医药卫生—诊断学] R734.2[医药卫生—临床医学]