组织工程学血小板裂解液促进骨重建实验研究  

Experimental study on tissue engineering platelet lysates in the promotion of bone reconstruction

作　　者：宋会平[1] 王志强[1] 李琪佳[2] 李宝兴[3] 白俊清[1] 

机构地区：[1]华北煤炭医学院附属医院骨科中心河北省创伤研究所,唐山063000 [2]华北煤炭医学院实验中心 [3]山西省医用组织库

出　　处：《中华医学杂志》2009年第43期3083-3086,共4页National Medical Journal of China

摘　　要：目的探讨组织工程学血小板裂解液（PL）对骨重建的干预效应。方法将制备的同种异体PL、同种异体脱钙骨颗粒（ADBG）与I型胶原凝胶（collagen type I，CG）复合构建重组合材料后，取成年健康Wistar大鼠30只，制双侧股骨髁缺损，分A、B、C3组（10只／组），分别植入等量PL／ADBG／CG、ADBG／CG和CG，移植4周时经放射学、组织学及组织形态计量学、生物力学和免疫学检测，评价骨缺损的重建效果。结果（1）X线显示A组修复区阻射影密度接近正常骨质，骨密度计量分析显示A组（4．2±1．0），显著高于B（2．4±0．9）和C组（1．1±0．5），差异有统计学意义（P〈0．01）；（2）组织学观察发现A组修复区有较多新生骨或移植骨片成活，无明显的淋巴细胞浸润，成骨活动和移植骨片破骨细胞性骨吸收均较B和C组明显活跃；形态计量分析显示A组单个视野的成骨面积（286．7±17．2）显著大于B（94．3±33．6）和C组（19．1±14．5），差异有统计学意义（P〈0．01）；（3）抗压生物力学测试结果：破断载荷A、B、C、正常对照组分别为（259．6±34．6）、（187．9±21．1）、（91．3±26．6）、（311．94±82．4）；破断能量A、B、C分别为（10．8±1．4）、（7．8±0．9）、（3．8±1．1）和（13．0±3．4）；破断载荷和能量A组均显著高于B和C组，但低于正常对照，差异有统计学意义（P〈0．01）；（4）三色流式细胞术分析显示，T细胞亚群CD3^＋CD4^＋CD8^-、CD3^＋CD8^＋CD4^-和CD4／CD8在各组间差异均无统计学意义（均P〉0．05）。结论组织工程学PL（PL／ADBG／CG）可加速骨缺损再生修复，并促进异体骨移植后骨吸收，从而加速骨整合重建。Objective To evaluate the effects of tissue engineered allogeneic platelet lysates （PL） upon bone reconstruction. Methods After preparation of recombinant material with PL, allogeneic decalcified bone granules （ADBG） and collagen type I （CG）, 30 healthy Wistar rats were used to prepare the bilateral bone defects in femoral condyles. The defects were filled with equivalent PL/ADBG/CG, ADBG/CG and CG in different groups of A, B and C （ with 10 rats each ） respectively. At 4 weeks, the defect reconstruction was evaluated with radiology, histology, immunology and biomechanics. Results （ 1 ） The X-ray showed that bone density in group A （4. 18 ± 0. 96） was close to that of normal hone and it was significantly higher than that in group B （2. 36 ± 0. 87 ） and group C （ 1.09 ± 0. 55 ） （ P 〈 0. 01 ）. （2） In comparisons with B and C, the histological assay revealed that there were markedly more activities of new bone formation and more implanted bone granules surviving without significant lymphocyte infiltration, as well as more osteoclastic bone resorption in group A. The bone histomorphometric assay showed the newly formed bone area in group A （ 286. 73 ± 17. 22 ） was significantly higher than that in group B （ 94. 34 ± 33.56） and group C （19. 12 ±14. 53） （P 〈0. 01 ）. （3） Anti-press mechanical measures showed that the destructive load in A, B, C and normal control group was 259. 63 ±34. 57, 187.90 ±21.07, 91.33 ±26. 58 and 311.93 ±82.45 respectively. The destructive energy in A, B, C and normal control group was 10. 82 ± 1.44, 7.83 ± 0. 88, 3.81 ± 1.11 and 12.97 ± 3.43 respectively. These results showed either destructive load or destructive energy in group A was markedly higher than that in group B and group C with significant difference （ P 〈 0. 01 ）, but still lower than that in normal controls （ P 〈 0. 01 ）. （ 4 ） Three-color flow cytometry assay showed that the T lymphocyte subsets of CD3 ^＋ CD4 ^＋ CD8^ - , CD3^ ＋ CD8

关 键 词：骨重建 血小板 移植 同种 

分 类 号：R686[医药卫生—骨科学]