代谢型谷氨酸受体激动药DHPG对大鼠海马穿通通路长时程抑制的影响  

Effect of Metabotropic Glutamate Receptors Agonist DHPG on Long-term Depression in the Hippocampal Perforant Pathway of Rats

作　　者：何蓉[1] 王兴勇[1] 肖敏意 熊燚[1] 周海银[1] 丁淼[1] 邱春兰[1] 许峰[1] 

机构地区：[1]重庆医科大学附属儿童医院,重庆市400014 [2]哥德堡大学,瑞典

出　　处：《中国药房》2009年第34期2649-2651,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30772362)

摘　　要：目的:观察代谢型谷氨酸受体(mGluRs)激动药3,5-二羟基苯甘氨酸(DHPG)对大鼠海马CA1区穿通通路(PP通路)诱发长时程抑制的作用及机制。方法:制作Wistar大鼠海马组织脑片,分为DHPG组、DHPG+N-甲基-D-天冬氨酸(NMDA)受体拮抗药AP5组和DHPG+双脉冲刺激(PPR)组(n分别为8、9、8),各组加入相应药物及操作后采用电生理记录加药前、后的场电位的标准化后均值。结果:DHPG组、DHPG+AP5组、PPR组加药前、后场电位均值分别为1.0049±0.101、0.7904±0.02197(n=8,t=15,P<0.01),1.0224±0.0724、0.828±0.01475(n=9,t=15,P<0.01),0.9525±0.1691、1.0511±0.2934(n=8,t=5,P<0.01)。结论:DHPG可诱发大鼠海马长时程抑制,且其属于mGluRs依赖型而非NMDA受体依赖型,并通过突触后诱发所致。OBJECTIVE： To investigate the mechanism of the inductive effect of metabotropic glutamate receptors （GluRs） agonist DHPG on long- term depression in hippocampal CA1 perforant pathway of rats. METHODS： Hippocampal tissue slices of Wistar rats were prepared and divided into three groups： DHPG group （n ： 8）, DHPG ＋ NMDA receptor antagonist AP5 group （n ~ 9）, and DHPG ＋ paired-pulse stimulation （PPR） group （n ： 8）. The standardized mean values of the field potential before and after drug treatment were recorded with the use of electrophysiological technology. RESULTS： The mean values of the field potential before vs. after drug treatment in the three groups were 1.004 9± 0.101 vs. 0.790 4 ± 0.02197（n=8, t =15, P〈0.01）, 1.0224±0.0724vs. 0.828±0.01475（n=9, t ：15, P〈0.01）, and0.9525±0.1691 vs. 1.051 1± 0.293 4 （n = 8, t= 5, P〈0.01）, respectively. CONCLUSION： DHPG can induce hippocampal long - term depression in mGluRs- dependent manner rather than NMDA receptor- dependent manner via a postsynaptic pathway.

关 键 词：3 5-二羟基苯氨酸 大鼠海马组织 长时程抑制 穿通通路 场电位 

分 类 号：R965[医药卫生—药理学]