紫外分光光度法测定IL-2靶向5-FURP脂质体中IL-2的含量  

Determination of IL-2 Content in IL-2 Targeting 5-FURP Liposomes by Ultraviolet Spectrophotometry

作　　者：吴镁春[1] 赵怡[1] 曾里[1] 王驰[1] 

机构地区：[1]重庆医科大学药学院,重庆市400016

出　　处：《中国药房》2009年第34期2701-2702,共2页China Pharmacy

摘　　要：目的:建立测定白细胞介素-2(IL-2)靶向5-氟尿苷棕榈酸酯前药(5-FURP)脂质体中IL-2含量的方法。方法:在样品溶液中加入考马斯亮蓝G-250溶液染色,以空白脂质体为空白,在595nm波长处照分光光度法测定IL-2的吸光度,并计算含量。结果:IL-2检测浓度的线性范围为1～100μg·mL-1(r=0.9997);平均回收率为99.26%,RSD=0.59%(n=5)。结论:本方法操作简便、快速、检测灵敏、重复性好,可用于该脂质体中IL-2的含量测定。OBJECTIVE： To determine the content of IL - 2 in IL - 2 targeting liposomes of 5 - FURP. METHODS： The sample solution was stained by Coomassie brillant blue G 250 solution. Using blank liposome as blank, the absorbability of the IL - 2 was determined by ultraviolet spectrophotometry at a wavelength of 595 nm; meanwhile its content was determined. RESULTS： The linear range of IL - 2 was 1-- 100μg·mL-1（r = 0.999 7） and the average recovery rate was 99.26% （RSD = 0.59%, n = 5）. CONCLUSION： The method was proved to be simple, rapid, sensitive and reproducible, and it is applicable for the determination of IL- 2 in liposome of 5-FURP.

关 键 词：白细胞介素-2 考马斯亮蓝G-250 5-氟尿苷棕榈酸酯前药 含量测定 

分 类 号：R927.2[医药卫生—药学]