微囊藻毒素LR对人FL和HL7702细胞PP2A各亚基转录水平的影响  

Effect of Microcystin LR on the Transcription of Subunits of PP2A of FL and HL7702

作　　者：梁婧[1] 黄朴[1] 傅文宇[1] 徐立红[1] 

机构地区：[1]浙江大学医学院生物化学与遗传学系,浙江杭州310058

出　　处：《癌变．畸变．突变》2009年第6期422-425,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(30771827);浙江省自然科学基金项目(Y206088)

摘　　要：背景与目的:研究微囊藻毒素LR(microcystin LR,MCLR)对正常人羊膜细胞FL和肝实质细胞HL7702蛋白磷酸酶2A(PP2A)各亚基mRNA表达的影响。材料与方法:FL和HL7702经100 nmol/L MCLR作用24 h后,采用实时定量PCR方法检测PP2A各亚基mRNA表达的变化。实验并设溶剂对照组。结果:FL细胞PP2A各亚基mRNA表达部分上升,部分下降;而HL7702细胞PP2A各亚基mRNA表达均上升。结论:MCLR对肝来源细胞的特异性作用可能与其肝脏毒性有关。BACKGROUND AND AIM： To investigate the effects of microcystin LR on the mRNA expression of protein phosphotase 2A subunits of human amniotic （FL） ceils and human hepatocyte HL7702 cells. MATERIALS AND METHODS： After treated with 100 nmol/L MCLR for 24 h, the mRNA expression of protein phosphotase 2A subunits of human anmiotic cells and human hepatocyte cells was measured by real time PCR. RESULTS： The changes of subunits expression were diverse in human amniotic cells but increased significantly in human hepatocyte cells. CONCLUSION： The special effects of MCLR on HL7702 cells could be account for its hepatotoxicity.

关 键 词：蛋白磷酸酶2A 微囊藻毒素 mRNA 实时定量PCR 

分 类 号：X503.1[环境科学与工程—环境工程]