铜缺乏状态下铜摄入量及铜锌比值对大鼠脂质过氧化的影响  

Effects of Copper Intake and Copper-zinc Ratio on Rat Lipid Peroxidation in Copper Deficiency

作　　者：段链[1] 程义斌[1] 金银龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100020

出　　处：《癌变．畸变．突变》2009年第6期447-451,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国际铜业协会(ICA)资助项目(H-AS-07-02)

摘　　要：背景与目的:探讨铜缺乏状态下,大鼠血中铜浓度及铜锌比值(Cu/Zn)与脂质过氧化各相关指标之间的关系,研究微量元素铜对大鼠脂质过氧化的影响。材料与方法:在铜缺乏状态下测定大鼠血中铜含量及血中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-pX)、过氧化氢酶(CAT)活性水平及丙二醛(MDA)含量。结果:42只SD大鼠随机分为正常对照组和6个实验组。对照组大鼠饲以正常饲料,实验组大鼠饲以缺铜饲料,同时灌胃给予不同剂量葡萄糖酸铜(Cu-G),各剂量实验组大鼠血中铜浓度呈现无规律波动,而Cu/Zn比值随着铜摄入量的增加呈递增趋势,差异有统计学意义(P<0.01)。在铜缺乏状态下,大鼠血中SOD活力水平低于正常水平,随着铜摄入量的增加,血中铜锌比值升高,SOD活力水平呈现上升趋势。铜缺乏时血中MDA含量升高,而随着铜摄入量的增加,即铜锌比值的升高,MDA含量有所下降,并保持在较低水平。血中铜锌比值的下降可能导致大鼠血中CAT活性水平的升高,但铜锌比值与CAT活性的相关关系呈非线性相关。铜缺乏及铜摄入量对大鼠血中GSH-pX无明显影响。结论:在研究微量元素铜的生理生化作用时,对铜锌比值(Cu/Zn)进行研究更具有实际意义。在铜缺乏状态下,大鼠血中SOD活性水平下降,同时血中MDA含量上升,而随着血中铜锌比值的升高,SOD活性呈上升趋势,MDA含量下降到较低水平。而铜摄入不足会使血中CAT的活性水平升高,对GSH-pX活性水平无明显影响。BACKGROUND AND AIM： To study the effect of trace element copper on lipid peroxidation by analyze relations among copper concentration, copper-zinc ratio （Cu/ Zn） and the biomarkers of lipid peroxidation in copper deficiency in rats. MATERIALS AND METHODS： Determined the content of copper in blood, the activities of Superoxide Dismutase （SOD）, Glutathione Peroxidase （GSH-pX） and Catalase （CAT） and the content of Malondialdehyde （MDA） in copper deficiency. RESULTS： Copper-defficient rats treated with copper gluconate （Cu-G） in different doses showed fluctuating copper concentrations in the blood, while copper-zinc ratio （Cu/Zn） showed an ascending trend following the increase of copper intake and statistical significant results （P 〈 0.01）. In copper deficiency state, the SOD activity was lower than the normal level, and then followed an upward trend with increased copper intake, equivalent to the copper-zinc （Cu/ Zn） ratio. The content of MDA was higher than the normal level, and its content decreased with increasing copper intake and the Cu/Zn ratio, and remained at a relatively low level. The decreased Cu/Zn ratio could induce the increase of the CAT activity, but with no linear correlation between them. No significant effect on the GSH-pX activity in rat blood was found during inadequate copper intake. CONCLUSION： Cu-Zn ratio has more practical significance in the research of physiological and biochemical roles of the trace element copper. In copper deficiency, the SOD activity in the rat blood was lower than the normal level and the content of MDA higher. With the increase of Cu/ Zn ratio, the SOD activity increased gradually, while the content of MDA was reduced. Insufficient copper intake could enhance the CAT activity in blood with no effect on GSH-pX.

关 键 词：铜 铜缺乏 脂质过氧化 超氧化物歧化酶 谷胱甘肽过氧化物酶 过氧化氢酶 丙二醛 

分 类 号：R591.1[医药卫生—内科学]