中药龟板对Parkinson病大鼠模型BMP4表达的影响  

Effects of plastrum testudinis on the expression of bone morphogenetic protein-4 in rat model of Parkinson's disease

作　　者：赵丹[1] 陈东风[2,3] 李伊为[2] 黎晖[2] 孟令杰[2] 周健洪[2] 邓汝东[2] 张瑞[2] 

机构地区：[1]海南医学院解剖学教研室,海口571101 [2]广州中医药大学解剖学教研室,广州510006 [3]南京大学医药生物技术国家重点实验室,210093

出　　处：《神经解剖学杂志》2009年第6期655-658,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30472272;30772861)资助项目

摘　　要：为研究益肾中药龟板对Parkinson病(PD)模型大鼠骨形成蛋白4(BMP4)表达的影响,本研究将6-羟基多巴胺注入大鼠黑质建立PD大鼠模型,对模型大鼠行高、低剂量龟板水煎液口服治疗45d后,采用ELISA和RT-PCR方法进行血清中BMP4酶联免疫试验和脑黑质、纹状体中BMP4mRNA的检测。结果显示:PD模型组的BMP4表达明显降低,用龟板治疗后可以显著阻碍此趋势,而且高剂量组比低剂量组效果更为明显(P<0.05)。本研究结果提示,龟板有明显的促进BMP4及BMP4mRNA表达的作用。To observe the effect of plastrum testudinis （PT） on the expression of bone morphogenetic protein-4 （BMP4） in rat model of Parkinson＇s disease（PD）. 6-OHDA（6-hydroxyl dopamines） was stereotactically injected into the rats substantia nigra to induce a rat model of PD. Oral administration of plastrum testudinis were conducted using higher dose and lower dose for 45 days. ELISA and RT-PCR method were used to detect the expression of the BMP4 in the substantia nigra and corpus striatum of PD rats. The results showed that the expression of BMP4 was reduced in the rat model of PD, but PT treatment reversed the declining tendency with more obvious effect appearing in higher dose group than lower dose group （P 〈 0.05 ）. The results suggest that plastrum testudinis can promote the expression of BMP4 and BMP4 mRNA in PD rats.

关 键 词：龟板 PARKINSON病 骨形成蛋白4 大鼠 

分 类 号：R285.5[医药卫生—中药学]