氧化修饰 LDL 对体外培养的主动脉壁内皮细胞的影响  

Effects of Oxidative Modified Low Density Lipoprotein on Cultured Aortic Endothelial Cells

作　　者：和红[1,3] 傅中滇[1] 蔡梅雪[1] 王福俤[2] 

机构地区：[1]上海基础医学院预防医学教研室 [2]第二军医大学海医系军队卫生学教研室 [3]上海第二医科大学卫生事业管理系

出　　处：《上海铁道大学学报》1998年第9期1-5,共5页

基　　金：国家自然科学基金

摘　　要：目的探讨氧化修饰低密度脂蛋白（ｏｘｉｄａｔｉｖｅｌｙｍｏｄｉｆｉｅｄｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ｏｘＬＤＬ）导致动脉粥样硬化形成的途径及可能机制。方法在体外培养的小牛主动脉内皮细胞（ｂｏｖｉｎｅａｏｒｔｉｃｅｎｄｏｔｈｅｌｉａｌｃｅｌ，ＢＡＥＣ）中加入ｏｘＬＤＬ，终浓度为０．１ｍｇＰｒ／ｍｌ，培养２４ｈ后，采用细胞毒试验、流式细胞仪、丙二醛（ＭＤＡ）测定及细胞计数等方法分别对其贴壁细胞及培养液进行了检测。结果加ｏｘＬＤＬ组与对照组（未加ｏｘＬＤＬ组）相比，发现：①在相差显微镜下，ｏｘＬＤＬ组细胞形态明显拉长，发生脱落，并存在大片脱失区；②细胞毒试验发现ｏｘＬＤＬ对ＢＡＥＣ的生长增殖有明显的抑制作用；③流式细胞仪检测发现ｏｘＬＤＬ抑制ＢＡＥＣ由Ｇ１期进入ＤＮＡ合成的Ｓ期，且ｏｘＬＤＬ可诱导ＢＡＥＣ凋亡的发生；④ＭＤＡ检测发现ｏｘＬＤＬ促进ＢＡＥＣ脂质过氧化物生成；⑤加ｏｘＬＤＬ组ＢＡＥＣ粘附的ＨＬ６０细胞数明显多于对照组。结论由ｏｘＬＤＬ作用引起的内皮细胞形态损伤、增殖抑制、脂质过氧化物生成增多及单核细胞粘附性增加等改变可能是动脉粥样硬化形成的机制之一。Objective To study the possible mechnism of the effects of oxLDL on the formation of atherosclerosis (AS).Methods Bovine aortic endothelial cells (BAEC) were cultured and treated with a concentrated (0.01mgPr/ml) oxLDL for 24 h.The cultured cells and the contents in the medium were studied by MTT colorimetric assay, flow cytometric method(FCM), TBA method, cell calculating and so on.Results (1) BAEC were deformed and shed as observed under phase contrast microscope in oxLDL group; (2) oxLDL had an inhibitory effect on the proliferation of BAEC; (3) oxLDL prevented BAEC from GI phase into S phase in DNA synthesis and induce apoptosis of BAEC; (4) MDA content in culture media in oxLDL group was significantly higher than that of control group; (5) The number of HL60 monocyte cells adhered to BAEC in oxLDL group was more markedly higher than that of control group.Conclusion In this experimental model study, oxLDL was proved having a definite harmful effect on the aortic endothelial cells and this initial damage might play a very important role in the process of AS formation.

关 键 词：内皮细胞 动脉粥样硬化 OXLDL 氧化修饰 

分 类 号：R543.5[医药卫生—心血管疾病]