妊娠期糖尿病患者腹部皮下脂肪组织胰岛素受体底物表达及其酪氨酸磷酸化的变化  

Tyrosine phosphorylation and expression of insulin receptor substrates in abdominal subcutaneous adipose tissues of patients with gestational diabetes mellitus

作　　者：朱颖[1] 朱旋[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉市430022

出　　处：《实用医学杂志》2009年第23期3962-3965,共4页The Journal of Practical Medicine

摘　　要：目的:探讨胰岛素受体底物(insulin receptor substrate,IRS)表达及其酪氨酸磷酸化的变化在妊娠期糖尿病(GDM)发病机制中的作用。方法:收集40例正常妊娠妇女和39例GDM患者的腹部皮下脂肪组织,分别用RT-PCR技术和Western blot印迹法检测组织中IRS-1和IRS-2 mRNA及其蛋白质的表达,同时测定患者空腹血糖、空腹胰岛素、体质指数等临床指标。结果:与正常妊娠妇女比较,GDM患者不仅存在明显的胰岛素抵抗(P<0.05),而且其腹部皮下脂肪组织中的IRS-1 mRNA和蛋白质的表达水平均显著降低,其酪氨酸磷酸化程度亦显著减弱,但是IRS-2 mRNA和蛋白质的表达水平均显著升高,其酪氨酸磷酸化程度亦显著增强,差异均有统计学意义(P<0.05)。结论:脂肪组织中IRS-1表达及其酪氨酸磷酸化水平减弱参与了胰岛素抵抗的发生,与GDM的发病关系密切。IRS-2表达及其酪氨酸磷酸化水平的增强不能完全代偿IRS-1信号缺陷。Objective To investigate the role of tyrosine phosphorylation and expression of insulin receptor substrates （IRS）in pathogenesis of gestational diabetes mellitus （GDM）. Methods Abdominal subcutaneous adipose tissues of 40 normal pregnant women and 39 GDM patients were collected. Expressions of IRS-1, IRS-2 mRNA and proteins were assessed by RT-PCR and Western blot respectively. Fasting blood glucose, fasting insulin and body mass index were determined. Results Compared with the normal pregnant women, the GDM patients had significantly insulin resistance （P 〈 0.05）, meanwhile, the expressions of IRS-1 mRNA and the protein in GDM patients were significantly decreased, which tyrosine phosphorylation was also significantly depressed, but the expressions of IRS-2 mRNA and the protein in GDM patients were significantly increased,which tyrosine phosphorylation was also significantly enhanced. The differences all reached statistical signifieanee（P 〈 0.05）. Conclusions The depression of IRS-1 expression and its tyrosine phospborylation participates in the development of insulin resistance and GDM,tbe reinforcement of IRS-2 expression and its tyrosine phosphorylation couldn＇t completely compensate IRS-1 signal flaw.

关 键 词：糖尿病 妊娠 胰岛素受体底物 脂肪组织 胰岛素抵抗 酪氨酸磷酸化 

分 类 号：R714.2[医药卫生—妇产科学] R587.1[医药卫生—临床医学]