石英微通道内测量细胞表面电荷的研究  

Study on measuring surface-charges of cells in a quartz microchannel

作　　者：陈德良[1] 冯彩玲[1] 秦水介[1] 

机构地区：[1]贵州大学贵州省光电子技术与应用重点实验室,贵阳550025

出　　处：《激光技术》2009年第6期561-564,共4页Laser Technology

基　　金：国家自然科学基金资助项目(60666001);贵州省科学技术基金资助项目(黔科合J字[2007]2201)

摘　　要：为了具体测量细胞表面电荷量,设计了一种利用光镊、微通道和电泳测量细胞表面电荷的方法,并利用该方法测量得到酵母菌细胞表面电荷大约为10^(-10)C。结果表明,可以利用该方法测量细胞表面电荷变化。对测量系统进一步开发,可做成测量细胞表面电荷的生物分析芯片。The surface-charges of cells are very important to study the vital characteristic of ceils. Common methods only measure the changes of cell electrophoresis mobility （EPM） instead of the quantity of cell surface-charges. In order to measure the surface-charges of cells, a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed. With this method, the quantity of the electric charge of yeast cells was measured about 10^-10C. Experimental results prove that this method can be used to measure the changes of cell surface-charges. A lab-onchip （LOC） for measuring the surface-charge of cells might be developed after improving this measuring system.

关 键 词：医用光学与生物技术 细胞表面电荷 光镊 石英微通道 电泳 

分 类 号：Q631[生物学—生物物理学] Q66