仲丁灵乳油高效液相色谱分析方法研究  

Analytical Method of Butralin in Emulsifiable Concentrate Using High Performance Liquid Chromatography

作　　者：吴小毛[1,2] 李明[1] 龙友华[1,2] 李荣玉[1] 

机构地区：[1]贵州大学作物保护研究所,贵阳550025 [2]贵州大学烟草重点实验室,贵阳550025

出　　处：《山地农业生物学报》2009年第5期432-435,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州大学自然科学青年基金项目资助[贵大自青基合字(X082057)];铜仁地区烟草公司项目资助[贵铜烟(2009)10号];贵州省烟草专卖局科技专项基金项目资助[(2007)07]

摘　　要：采用高效液相色谱法,使用SunFireTMC18不锈钢色谱柱和紫外检测器,以乙腈∶水(80∶20,v/v)为流动相,流速1.2 mL.min-1,在240 nm波长下,对仲丁灵乳油进行定量分析测定。结果表明,仲丁灵的线性相关系数为0.9998;标准偏差为0.021;变异系数为0.06%;平均回收率为99.6%。该方法操作简便、快速、准确,重现性好,可供仲丁灵乳油质量控制用。The quantitative analysis method of butralin in emulsifiable concentrate was developed by Wasters 600E High Performance Liquid Chromatography （HPLC） consisting Waters 2487 ultraviolet absorbance detector in the current case. The analytical column was a SunFireTM C18 column, 150 ×4.6 mm. The mobile phase was acetonitrile -water （80： 20, v/v） at a flow rate of 1.2 mL·min^-1. The wavelength of detector was set at 240 nm. The results showed that the linear correlation coefficient of the analytical method for butralin was 0.999 8, and the standard deviation was 0. 021. The coefficient of variation was 0.06% , and the average recovery ratio was 99.6%. The method was proved to be simple, rapid, precise, and reproducible, and can be used for quality detection of butralin in emulsifiable concentrate.

关 键 词：仲丁灵 高效液相色谱法 定量分析 

分 类 号：S482.892[农业科学—农药学]