腹透液相关浓度葡萄糖对人腹膜间皮细胞端粒长度的影响及葛根素的拮抗作用  

Effect of Glucose Related to Peritoneal Dialysate on Telomere Length in Human Peritoneal Mesothelial Cells and Protection of Puerarin

作　　者：曹东维[1] 张苗[1] 蒋春明[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肾科,江苏南京210008

出　　处：《现代生物医学进展》2009年第19期3716-3717,共2页Progress in Modern Biomedicine

基　　金：南京市医学科技发展重点项目(ZKX0416)

摘　　要：目的:研究腹透液相关浓度葡萄糖(1.5%、2.5%)对人腹膜间皮细胞端粒长度的影响及葛根素的拮抗作用。方法:人腹膜间皮细胞在含糖(1.5%、2.5%)、含糖含葛根素(葛根素终浓度为100μg/ml)的无血清培养基培养24h后,收集细胞,提取DNA,采用Southern杂交测定细胞端粒长度。结果:经含糖1.5%和2.5%培养基培养24h后腹膜间皮细胞大多出现肥大。高浓度葡萄糖组细胞端粒长度缩短,2.5%组和1.5%组无显著差别(P>0.05)。葛根素加入后含糖组腹膜间皮细胞端粒长度延长(P<0.05)。结论:高糖可使间皮细胞端粒缩短,且可能与高糖作用下细胞肥大有关,葛根素可拮抗高糖对间皮细胞端粒长度的影响。Objective：To investigate the effect of different concentrated glucose on telomere length in cultured human peritoneal mesothelial cell（HPMC） and Protection of Puerarin.Methods： HPMC were cultured with serum free RPMI 1640 medium containing different glucose concentrations（1.5% and 2.5%）and Puerarin（100μg.ml-1） for 24 hours.Iisolated the DNA,telomere length was detected by Southern blot.Results： In the presence of 1.5% and 2.5% glucose concentrations,most of the cells became hypertrophic. Telomere length of the groups of high glucose were shortened, and there were no difference between the 1.5% and 2.5%（ P 〉 0.05）. Telomere length shortening were decreased in groups of Puerarin. Conclusion： High concentrated glucose stimulated the shortening of telomere length, which might be related to the hypertrophy. And Puerarin might resist the effect.

关 键 词：腹膜间皮细胞 葡萄糖 端粒 葛根素 

分 类 号：R69[医药卫生—泌尿科学] R285.5[医药卫生—外科学]