毛细管电泳电化学免疫分析法检测人血清乙肝表面抗原  

Application of capillary electrophoresis electrochemical immunoassay in determination of HbsAg in human serum

作　　者：陈钰[1,2] 吴国权[1] 刘仲明[1] 王捷[1] 

机构地区：[1]广州军区广州总医院医学实验科,广东广州510010 [2]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代生物医学进展》2009年第19期3738-3740,3715,共4页Progress in Modern Biomedicine

基　　金：2006年广东省自然科学基金重点项目"骨唾液蛋白在乳腺癌亲骨转移中的作用"(编号:06104396)

摘　　要：目的:建立用于测定人血清中乙肝表面抗原的毛细管电泳电化学免疫技术。方法:以微量滴定板为固相载体,预包被上乙肝表面抗体(抗-HBs),与血清乙肝表面抗原(HBsAg)及活化钌标记的乙肝表面抗体之间发生特异性免疫反应,形成三明治结构,将此双抗夹心免疫复合物进行毛细管电泳电化学分析。结果:利用此方法测定人乙肝表面抗原含量,浓度在0.08 ng/mL^10 ng/mL范围内与峰电流呈良好的线性关系,线性回归方程为y=0.2840x+0.9535,检出限为0.01 ng/mL(3σ)。结论:该法可以实现低浓度乙肝表面抗原血清样本低廉、快速、简便、定量检测。Objective：To develop an capillary electrophoresis electrochemical immunoassay strategy for the determination of HBsAg in human serum.Methods： Specific immunological reaction was take place at microtiter plate,between the pre-coated anti-HBs,human HBsAg and Ru（bpy）32＋-labled anti-HBs,which formed sandwich-typed immunocomplex.Then the target HBsAg was determined by Capillary electrophoresis electrochemical immunoassay.Results： The protocol has been characterized with cyclic voltammogram and potentiostatic method. A good linear relationship between peak current and the HBsAg concentration of 0.08 ng/mL-10 ng/mLwas obtained, and the detection limit was 0.01 ng/mL （3σ）. Conclusion： The protocol could realize cheap, rapid, simple, convenient and quantitative determination of low concentration HBsAg in human serum.

关 键 词：乙肝表面抗原 毛细管电泳 电化学免疫检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] R319[自动化与计算机技术—控制科学与工程]