不知火杂柑叶片黄化对其光合特性的影响  

The Effects of Leaf Chlorosis of Shiranuhi on its Photosynthetic Characteristic

作　　者：齐笑笑[1] 胡娟[1] 肖家欣[1] 周守标[1] 申燕[1] 杨慧[1] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全安徽省高校省级重点实验室,安徽芜湖241000

出　　处：《中国农学通报》2009年第22期217-220,共4页Chinese Agricultural Science Bulletin

基　　金：安徽省自然科学基金(070411004);中国博士后基金(20080430172);安徽师范大学重点科研项目(2008Xzd004)

摘　　要：为了探究不知火杂柑叶片黄化特征及其对光合作用的影响,运用CI-340便携式光合测定仪对田间的正常与黄化不知火杂柑的光合特性进行了测定,并通过扫描电镜观察了叶表皮特征,结果表明:黄化不知火杂柑叶片的叶绿素a+b含量、叶面积显著低于正常不知火(P=0.045,P=0.003),其类胡萝卜含量却显著高于正常不知火(P=0.049);二者气孔均分布于下表皮,上午8:00二者间的气孔密度与气孔开度并无明显差异。两者净光合速率(Pn)日变化均呈现出"单峰"曲线,正常不知火杂柑的Pn高峰出现在8:00,较黄化不知火杂柑早2 h,其中黄化不知火杂柑Pn峰值相对较低。简单相关分析结果表明,对黄化不知火杂柑Pn影响显著的因子依次是气孔导度(Gs)、田间CO2浓度(Ca)、光合有效辐射(PAR)和蒸腾速率(Tr);而与正常不知火杂柑Pn呈显著相关的因子是Gs和Tr。结合线性回归分析及偏相关系数表明,正常、黄化不知火杂柑的Pn日变化均与PAR呈极显著相关。In order to investigate the characteristic of chlorotic leaves from shiranuhi and its effects on photosynthesis, the photosynthetic characteristics of leaves from normal and ehlorotic shiranuhi under field conditions were determined using the CI-340 portable photosynthesis system, and the leaf epidermis features of them were observed by scanning electron microscope （SEM）. The results showed that ehlorotic leaf chlorophyll a＋b concentration and leaf area were significantly lower than those of normal shiranuhi （P=0.045, P =0.003）, whereas carotenoid concentration was dramatically higher in chlorotie leaves than in normal leaves from shiranuhi （P =0.049）, stomas were all distributed in lower epidermis of both, at a.m. 8：00, no distinct differences were de- tected on the stomatal frequency and apertures among them. The single peaks were found on the diurnal variations in net photosynthetic rate （P.） of them, the peak of normal shiranuhi occurred at 8：00, in advance two hours compared with that of chlorotic shiranuhi, furthermore, the peak of P, was relatively lower in leaves from ehlorotic shiranuhi than from the normal one. Simple correlation analyses showed that the factors affecting Pn of ehlorotic shiranuhi could be ranked, from the highest to the lowest, as stomatal conductance（G.）〉 CO2 concentration in field （C.） 〉photosynthesis availability radiation（PAR）〉 transpiration rate（Z）, however, Pn of normal shiranuhi was significantly correlated to Gn and Tr. The analysis of multiple-regression and partial correlation coefficient suggested that the diurnal variations of Pn of both normal and chlorotic shiranuhi had very significant correlation with PAR.

关 键 词：不知火杂柑 叶片黄化 光合特性 相关性 

分 类 号：S666[农业科学—果树学] Q945[农业科学—园艺学]