血透患者血清诱导内皮细胞血管样结构形成  

Long-term haemodialysis patients sera can induce tube formation of endothelial cells.

作　　者：王喜超[1] 涂阳科[1] 

机构地区：[1]天津市第一中心医院肾内科,天津300192

出　　处：《海南医学》2009年第12期23-24,共2页Hainan Medical Journal

摘　　要：目的比较健康志愿者血清、短期透析患者血清与长期透析患者血清体外诱导内皮细胞血管样结构形成的不同。方法用10%长期透析患者血清培养基(长期透析组)、10%短期透析患者血清培养基(短期透析组)及10%健康志愿者血清培养基(正常对照组)培养人脐静脉血管内皮细胞。倒置显微镜下观察血管样结构形成。结果长期透析组细胞培养30 h后,有血管样结构形成,平均面积(1438±112)μm2,而短期透析组与正常对照组无血管样结构形成。结论长期维持性血透患者血清能够诱导脐静脉内皮细胞血管样结构形成。Objective To compare tube formation of human umbilical vein endothelial cells （HUVECs） induced by sera medium between long - term haemodialysis, short - term haemodialysis patients and healthy volun-teers. Methods HUVECs are respectively cultured in 10% long - term haemodialysis patients sera medium （ long - term haemodialysis group）, 10% short- term haemodialysis patients sera medium （short- term haemodialysis group） and 10% healthy volunteers sera medium（ control group）. Tube formation are obsersed under light - miero- scope. Results There were tube formation in long - term haemodialysis group after endothelial cells are cultured for 30 hours, but tube formation were not found in short - term haemodialysis group and control group. Conclusion Long - term haemodialysis patients sera medium can induce tube formation of HUVECs.

关 键 词：血管新生 内皮细胞 血液透析 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学]