氟离子对西伯利亚鲟胚胎发育的影响  

Effects of fluoride ion(F^-) on embryo development of Siberian sturgeon,Acipenser baeri

作　　者：王瑞芳[1,2,3] 庄平[1,2,3,4] 章龙珍[1,3,4] 石小涛[1,2] 刘鉴毅[1] 张俊[4] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海200090 [2]华东师范大学生命科学学院,上海200062 [3]大连水产学院生命科学与技术学院,大连116023 [4]上海海洋大学水产与生命学院上海高校水产养殖E-研究院,上海201306

出　　处：《海洋渔业》2009年第4期357-362,共6页Marine Fisheries

基　　金：国家高技术研究发展计划资助项目(2008AA10Z227);浙江省科技计划项目(2007C12026);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目(2007M02);中国水产科学研究院内陆渔业生态环境和资源重点开放实验室开放基金(YM2007-06);华东师范大学2007年优秀博士生培养基金

摘　　要：本试验在水温17±1℃的条件下,采用半静态式生物毒性试验方法,探讨了氟离子(F-)对西伯利亚鲟胚胎发育的影响。结果表明,100~600 mg/L氟暴露会导致胚胎孵化延迟,氟暴露组半数孵化时间(MHT)比对照组推迟9~22 h。氟暴露同时导致胚胎死亡率显著增加(P<0.05),出膜仔鱼活力减弱、存活时间缩短、畸形率显著升高(P<0.05),出现卵黄囊畸形、脊椎畸形、眼部充血等畸形症状。试验得出F-致西伯利亚鲟胚胎144 h半致死浓度(LC50)为447.61 mg/L,孵化安全浓度(SC)为4.48 mg/L,导致仔鱼畸形的半数效应浓度(EC50)为536 mg/L。氟对西伯利亚鲟胚胎的安全质量浓度低于我国部分高氟地区地下水、地表水及人为氟污染水域中氟含量,提示天然水体中氟可能会对鱼类胚胎产生负面影响,应引起重视。Fluoride ions caused widespread endemic fluorosis disease to human beings. However, little attention was paid to the effect of fluoride ions on aquatic organisms. The aim of this study is to investigate the effect of fluoride ions on embryo development of Siberian sturgeon Acipenser baeri. In semi-static toxicity bioassays, Siberian sturgeon embryos （multi-cell stages ） were exposed to six different concentrations of waterborne fluoride （ 100 -600 mg/L） at 17 ± 1℃. Embryo development and hatched larvae were assessed in the experiment. Fluoride resulted in increased embryo mortality, decreased hatching rate, and increased deformed rate of larva compared with the control group. Fluoride caused 9 -22 hour extended development time （delayed hatching） to embryos, compared to the control group. 144 h median lethal concentration（ LC50 ） was 447.61 mg/L for Siberian sturgeon embryo, Safety Concentration （SC） of hatching was 4.48 mg/L, median effective concentration （EC50） of larva deformed was 536 mg/L. Study found that safety Concentration （SC） of hatching was lower than water fluoride concentration in high fluorine area and high-fluoride sewage, which reminds us that more attention should be paid to the effect of fluoride on aquatic organisms.

关 键 词：氟 西伯利亚鲟鱼 胚胎发育 延迟 安全浓度 

分 类 号：S917[农业科学—水产科学]