南通地区B型乙型肝炎病毒PreS基因参照序列的建立  

ESTABLISHMENT OF CONSENSUS SEQUENCE OF PRES OF HEPATITIS B VIRUS GENOTYPE B IN NANTONG CITY

作　　者：李海波[1] 强福林[2] 杨自力[1] 崔晓燕[1] 陈秀芬[3] 吴徐明[2] 

机构地区：[1]江苏省南通市疾病预防控制中心,南通226007 [2]江苏省南通市肿瘤医院 [3]江苏省南通市中医院

出　　处：《现代预防医学》2009年第23期4498-4500,共3页Modern Preventive Medicine

基　　金：江苏省预防医学科研课题(Y2006006);南通市社会发展科技计划项目(S2007013)

摘　　要：[目的]建立南通地区B型HBV流行株PreS基因参考序列。[方法]扩增HBV慢性无症状携带者的PreS基因,分析确定基因型并拟定参照序列。将所测基因序列与HBV不同型PreS序列用Clustalx1.8软件比对,经MEGA3.1分型,选取其中的B型HBV基因,建立南通地区B型HBV流行株的PreS参考序列。[结果]与GenBank收录的HBV-B型序列比较,核苷酸和氨基酸的同源率分别为98.5%～99.2%和96.6%～98.9%。[结论]成功建立了南通地区B型HBV流行株的PreS基因参照序列。[Objective]To develop consensus sequence of PreS of Hepatitis B virus genotype B in Nantong city. [Methods] The gene of HBV PreS was amplified with PCR and sequenced. The main epidemic strain of HBV genotype B was identified to establish its consensus sequence. [Results] The sequences of all samples were analyzed using Clustalx1.8 and MEGA3.1. The consensus sequence of PreS of HBV genotype B was established. Compared with other HBV PreS sequences, the homology rate of nucleotide and amino acid was 98.5%-99.2% and 96.6%-98.9% respectively. [Conclusion] The consensus sequence of PreS of HBV genotype B is established.

关 键 词：乙型肝炎病毒 PreS基因 参照序列 

分 类 号：R512.62[医药卫生—内科学]