鸡端粒酶RNA基因的克隆  

Cloning of Chicken Telomerase RNA Gene

作　　者：郑梅竹[1,2] 时东方[1] 潘风光[3] 艾永兴[2] 王秋悦[4] 刘春明[1] 

机构地区：[1]长春师范学院中心实验室,长春130032 [2]吉林大学畜牧兽医学院,长春130062 [3]吉林大学军需科技学院,长春130062 [4]河北科技师范学院动物科学系,秦皇岛066600

出　　处：《基因组学与应用生物学》2009年第5期865-868,共4页Genomics and Applied Biology

基　　金：国家自然基金资助项目(30471284)资助

摘　　要：本研究采用扩增条件优化的PCR扩增技术,以MDCC-MSB1细胞基因组DNA为模板扩增出鸡端粒酶RNA(chicken telomerase RNA,chTR)全长基因,克隆到pMD18-T载体中,经酶切鉴定和PCR鉴定后测定序列。序列分析表明所克隆的鸡端粒酶RNA基因全长465bp,其中模板区的11个核苷(5'-CUAACC-CUAAU-3')合成端粒亚单位(TTAGGG)n。chTR基因的克隆为进一步研究chTR在马立克氏病发病过程中的作用以及马立克氏病的发病机制提供可能的序列基础。The chicken telomerase RNA intact gene was obtained by optimizing PCR reaction system using MDCC-MSB 1 genomic DNA as a template, and cloned it into plasmid vector pMD18-T. The product of the PCR was analyzed by sequencing after identification by PCR and restriction enzyme digestion. The sequence analysis indicated that the full length of chicken telomerase RNA intact gene was 465 bp, in which there were 11 nucleotides, 5＇-CUAACCCUAAU-3＇, encompassed template region of chicken telomerase RNA and composed the subunits of telomerase （TTAGGG）,. The cloning of chicken telomerase RNA may offer some possible sequences for finding out the roles of chicken telomerase RNA in the process of Marek＇s disease virus and researching on the pathogenesis of Marek＇s s disease virus.

关 键 词：鸡端粒酶RNA基因 基因克隆 Touch-down PCR 

分 类 号：Q78[生物学—分子生物学]