华东竹黄菌不同居群遗传分化的RAPD分析  

Genetic Differentiation of Shiraia bambusicola Populations from East China Revealed by RAPD Analysis

作　　者：范英[1] 钟成刚[2] 程洁[1] 陈双林[1] 

机构地区：[1]南京师范大学生命科学学院,南京210046 [2]浙江工业大学药学院,杭州310014

出　　处：《基因组学与应用生物学》2009年第5期889-895,共7页Genomics and Applied Biology

摘　　要：为了解竹黄地理居群间的遗传分化,本研究采用随机引物扩增多态性DNA分子标记技术对江苏、安徽和浙江3省的8个居群共32个竹黄样本进行了遗传多样性分析。从50个RAPD引物中筛选得到了5个随机引物,对供试材料的DNA进行扩增,共检测出77个位点,其中多态性位点52个,多态性位点比率为67.53%。UPGMA聚类分析结果表明,这8个居群分为三类:安吉居群、临安居群、宜兴居群、广德居群和泾县居群聚为一类;宁国居群和休宁居群聚为一类;淳安居群单独为一类。遗传多样性分析表明8个竹黄居群中,淳安居群的遗传多样性水平最高,安吉居群的遗传多样性水平最低。Nei's基因多样性指数和Shannon信息指数均表明竹黄物种水平的遗传多样性高于居群水平。In this research, we have analyzed the genetic diversity of the 32 individuals in 8 populations collected from Jiangsu, Anhui and Zhejiang provinces by employing random amplified polymorphic （RAPD） DNA markers technique in order to clarify the genetic differentiation among different geographical populations of Shiraia bomb usicola. We acquired 5 random primers screened from 50 RAPD primers to identify the polymorphism among the tested materials. The results showed that 77 loci were detected, among which 52 were polymorphic loci accounted for 67.53% polymorphism. Cluster analysis by using unweighted pair group method arithmetic average （UPGMA） demonstrated that the 8 populations were mainly divided into three groups, among which population Anji （AJ）, population Linan （LA）, population Yixing （YX） and population Guangde （GD） were clustered in the same group and the second group included population Ningguo （NG） and population Xiuning （XN）, while population Chunan （CA） was the single one of the third group. Genetic analysis demonstrated that population Chunan （CA） had the highest genetic diversity level, while population Anji （AJ） had the lowest. Both Nei＇s gene diversity index and Shannon＇s information indexes indicated that the genetic diversity of Shiraia bambusicola was higher at the species level than that at the population level.

关 键 词：竹黄 遗传分化 RAPD 

分 类 号：S567.39[农业科学—中草药栽培]