HPLC法测定复方金霉素甘油中盐酸金霉素的含量  

Determination of Chlortetracycline Hydrochloride in Compound Chlortetracyctini Glycerin by HPLC

作　　者：焦志铁[1] 张树倩[1] 

机构地区：[1]江苏省扬州市第一人民医院,扬州225001

出　　处：《中国药事》2009年第11期1115-1116,1119,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法测定本院制剂复方金霉素甘油中盐酸金霉素的含量。方法采用Autima C18柱;流动相为甲醇-0.01mol.L-1NaH2PO4（以磷酸调节pH至3.3）（36∶64）;流速：1.0mL.min-1;检测波长：355nm。结果盐酸金霉素在85.56~513.36μg.mL-1范围内线性关系良好（r=0.9997）;平均回收率为100.23%,RSD为1.34%。结论本方法简便,快速,准确,重现性好,可用于复方金霉素甘油的质量控制。Objective To establish a method for determination of chlortetracycline hydrochloride in compound chlortetracyctini glycerin.Methods The method of HPLC was applied.The sample was separated on Autima C18 column with a mixture of methanol and 0.01mol·L-1 NaH2PO4 solution（36∶64） as the mobile phase.The UV detector wavelength was 355nm.Results The linearity was obtained within the range of chlortetracycline hydrochloride concentrations from 85.56μg·mL-1 to 513.36μg·mL-1,r=0.9997.The average recovery was 100.23%,, and RSD was 1.34%. Conclusions The method is simple, rapid, accurate and repeatable, which could be used to quality control of compound chlortetracyctini glycerin.

关 键 词：盐酸金霉素 复方金霉素甘油 HPLC 

分 类 号：R284.2[医药卫生—中药学] R927.2[医药卫生—中医学]