病原侵染后杨树叶片中两个关键酶的表达分析  

Expression Analysis of Two Key Enzymes in Poplar Leaves after Pathogen Infection

作　　者：袁坤[1] 张燕梅[2] 胥猛[3] 王明庥[3] 黄敏仁[3] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学重点开放实验室,海南儋州571737 [2]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [3]南京林业大学林木遗传与基因工程实验室,南京210037

出　　处：《中国农学通报》2009年第23期170-174,共5页Chinese Agricultural Science Bulletin

基　　金：国家高技术研究发展计划(2006AA100109)"高产优质多抗林木花草分子与细胞育种技术及品种创制;国家"十一五"科技支撑计划(2006BAD01A1503)"高产优质美洲黑杨速生材新品种选育"资助

摘　　要：抗坏血酸过氧化物酶(ascorbate peroxidase,APX)和S-腺苷甲硫氨酸合成酶(S-adenosyl methionine synthetase,SAMS)在植物胁迫应答中扮演重要角色。为了研究这两个酶与杨树抗病的关系,以杨树高抗无性系"NL895"(P.euramericana CL"NL895")为试验材料,利用实时定量RT-PCR(Real-time RT-PCR)技术,分析病原侵染不同时间后杨树叶片中这两个酶在mRNA水平上的表达变化,结果显示,这两个酶均差异表达且表达量下调,表明二者与杨树抗病过程是密切相关的。进一步将APX和SAMS在转录组水平上的表达变化与蛋白质组水平上的变化进行比较后发现,二者在两种水平上的表达变化无显著相关性。Ascorbate peroxidase （APX） and S-adenosyl methionine synthetase （SAMS） play important roles in plant stress response. To study the relations between the two enzymes and poplar disease resistance, the poplar high resistant clone ＂NL895＂ was used as the experimental materials and then the expression changes of them at mRNA level after different times of pathogen infection in poplar leaves were analyzed utilizing the real-time RT-PCR. The results showed that the two enzymes were both differential and dropping expression. It indicated that they were closely correlative with poplar disease resistance. It was found that the expression changes of APX and SAMS at transcriptome and proteome levels had no significant correlations by further comparing the expression ones.

关 键 词：APX SAMS 杨树 实时定量RT-PCR 差异表达 

分 类 号：S3[农业科学—农艺学]