焦虑对体液免疫功能影响及其与HLA-DQB1等位基因的关系  

Influences of anxiety on humoral immune functions and the correlations with HLA-DQB1

作　　者：高昆[1] 刘辉[1] 

机构地区：[1]大连医科大学检验医学院临床免疫教研室,大连116044

出　　处：《中国免疫学杂志》2009年第11期1045-1047,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金(30770724)项目

摘　　要：目的:研究焦虑对体液免疫功能的影响及其与HLA-DQB1等位基因多态性的关系。方法:随机挑选某医院健康体检住院医生31名,选用状态-特质焦虑量表(STAI)来测量其焦虑状况,实验室检测IgG、IgA、IgM以及补体C3、C4水平,并利用PCR扩增各研究对象HLA-DQB1*02、*03、*04、*05和*06五个位点基因多态性。结果:状态焦虑(Ta)和特质焦虑(Tc)均同补体C3呈正相关,有统计学意义。HLA-DQB1*02位点阳性和阴性的个体状态焦虑的差异有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P>0.05)。HLA-DQB1*04位点阳性和阴性的个体状态焦虑和特质焦虑的差异都有统计学意义(P<0.05),而补体C3的差异不具有统计学意义(P<0.05)。结论:焦虑可以引起某些体液免疫功能指标的改变并与HLA-DQB1等位基因表型有关。Objective： To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms. Methods： Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory （STAI）. IgG, IgA, IgM, complement C3 and complement C4 were detected with BECKMAN array360 system; HLA-DQB1 ＊ 02, ＊ 03,＊ 04, ＊05 and ＊ 06 alleles were individually amplified by polymerase chain reaction（PCR）using exon2 greup-specific primers. The correlation between immune function and HLA-DQB1 polgmorphisms were investigated. Results： Statistical analysis showed that there was positive correlation with State Anxiety （Ta） and complement C3, either Trait Anxiety （Tc） and complement C3. There was significant difference between HLA- DQB1 ＊ 02 positive and negative in Ta （P 〈 0.05）, while no difference in complement C3（ P 〉 0.05）. There was significant difference between HLA-DQB1 ＊ 04 positive and negative in Ta and Tc（ P 〈 0.05）, while no difference in complement C3（ P 〉 0.05）. Conclusion：Anxiety could change some humoral immune functions and this is related with HLA-DQBI polymorphism.

关 键 词：焦虑 体液免疫功能 HLA-DQB1 

分 类 号：R392[医药卫生—免疫学] R395[医药卫生—基础医学]