超声增强紫杉醇微泡剂对人卵巢癌COC1细胞增殖的抑制作用  

Ultrasound Enhancing Inhibition of the Proliferation of Human Ovarian Cancer Cells COC1 by Paclitaxel Microbubbles

作　　者：雷琪[1] 周溯[2] 甘强[1] 

机构地区：[1]重庆广播电视大学药学系,400052 [2]四川石油管理局总医院药剂科,成都610213

出　　处：《医药导报》2009年第12期1551-1553,共3页Herald of Medicine

摘　　要：目的探讨超声联合紫杉醇微泡剂对人卵巢癌细胞COC1的增殖抑制作用。方法将人卵巢癌细胞株COC1随机分为6组:对照组、空白微泡剂+超声组、紫杉醇微泡剂组、单纯紫杉醇组、紫杉醇+超声组、紫杉醇微泡剂+超声组。观察各组处理后癌细胞的增殖、细胞形态及细胞凋亡情况。结果紫杉醇微泡剂+超声组COC1细胞增殖被明显抑制,24,48,72 h的抑制率分别为(43.60±2.64)%,(37.89±1.30)%,(28.95±2.89)%。电镜观察结果显示,紫杉醇微泡剂+超声组的COC1细胞数量减少最显著(P<0.05),细胞折光度差,细胞固缩,出现许多细胞碎屑,PI/hoechst染色检测显示细胞凋亡率最高,为(52.67±9.29)%(P<0.05)。结论超声能有效增强紫杉醇微泡剂对人卵巢癌细胞COC1的杀伤能力。Objective To determine inhibition of the proliferation of human ovarian cancer cells COCI by ultrasonic irradiation and paclitaxel-carrying microbubbles. Methods The cells were randomly divided into six groups： control group, blank microbubbles plus ultrasound, paclitaxel-carrying microbubbles, paclitaxel, paclitaxel plus ultrasound, and paclitaxel-carrying microbubbles plus ultrasound. The inhibitory effect of the cell growth was observed by the MTT assay, cell morphology and apoptosis rate were detected. Results The growth of COC1 cells were significantly suppressed by paclitaxel-carrying microbubbles plus ultrasound, the inhibitory rates of which were （ 43.60 ± 2.64 ） %, （ 37.89 ± 1.30 ） %, （ 28.95 ± 2.89 ） % at 24,48,72 h, respectively. The most significant cell number reduction was found in the group of paclitaxel-carrying microbubbles plus ultrasound（P 〈 0.05 ） and the decreased cell diopter, cell shrinkage and a lot of cell debris were observed as well. The apoptosis rate reached （ 52. 67 ± 9. 29 ） % by PI/hoechst staining assay （ P 〈 0. 05 ）. Differences with other groups were significant. Conclusion Low-intensity focused ultrasound could effectively enhance inhibition of proliferation of human ovarian cancer cells COCI by paclitaxel-carrying microbubbles.

关 键 词：紫杉醇 微泡剂 卵巢癌 超声 

分 类 号：R979.1[医药卫生—药品] R737.31[医药卫生—药学]