丝裂霉素C联合原花青素对人膀胱癌BIU87细胞的生长抑制作用  

Inhibition of Combining Mitomycin C with Grape Seed Procyanidin Extract on the Growth of Human Bladder Cancer Cell Line BIU87

作　　者：刘洁[1] 庄乾元[1] 张维怡[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [2]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030

出　　处：《医药导报》2009年第12期1556-1558,共3页Herald of Medicine

摘　　要：目的探讨丝裂霉素C(MMC)与葡萄籽提取物原花青素(grape seed procyanidin extract,GSPE)联合应用对人膀胱癌BIU87细胞的生长抑制作用。方法采用噻唑蓝(MTT)比色法测定MMC和GSPE单独和联合应用时对人膀胱癌BIU87细胞生长的抑制作用,并应用Chou-Talalay联合指数法定量分析药物之间的相互作用效果。结果MMC、GSPE单独应用时,BIU87细胞生长的抑制作用随药物浓度的增加而增加,中效浓度分别为15.422,195.865μg.mL-1。MMC与GSPE联合应用时,作用效果表现为在低浓度(0%<fa<37%)时呈协同作用(CI<1),高浓度(37%<fa<100%)时呈拮抗作用(CI>1),中效浓度为109.496μg.mL-1(其中MMC 8.111μg.mL-1,GSPE101.385μg.mL-1)。结论MMC和GSPE相互作用的效果在低浓度时呈协同作用,高浓度时呈拮抗作用。Objective To explore the anti-cancer effects of mitomyein C （MMC） and grape seed procyanidin extract （GSPE） on human bladder cancer cell line BIU87. Methods The 3- [ 4,5-dimethyhhiazol-2-yl ]-2,5-diphenyltetrazolium （MTT） method was used to detect the inhibition rates of MMC and GSPE by single or combined usage, the combination index （CI） method of Chou-Talalay was used to quantitatively analyze the combined effects. Results The anti-cancer effects of MMC and GSPE were enhanced in a dose-dependent manner, respectively. The media-effect concentrations were 15. 422 and 195. 865 μg·mL^-1, respectively. MMC was synergetic with GSPE （CI 〈 1 ） at low concentrations （0% 〈 fa 〈 37% ）, while antagonistic （CI 〈 1 ） at high concentrations （37% 〈 fa 〈 100% ） when used combined with GSPE. The media-effect concentrations was 109. 496 μg·mL^-1 （ in which MMC was 8. 111μg·mL^-1 , GSPE 101. 385 μg·mL^-1 ）. Conclusion The combination effects of MMC and GSPE is synergetie at low concentrations, while antagonistic at high concentrations.

关 键 词：丝裂霉素 原花青素 癌 膀胱 

分 类 号：R979.1[医药卫生—药品] R737.14[医药卫生—药学]