黄芪注射液对阻塞性黄疸大鼠小肠黏膜的保护作用  

Protective Effect of Radix Astragali Injection on the Intestinal Mucosa of Rats with Obstructive Jaundice

作　　者：华育晖[1] 王刚[1] 余亚平[2] 孙国明[1] 骆英[1] 张喜平[3] 

机构地区：[1]杭州市第一人民医院药剂科,杭州310006 [2]浙江中医药大学,杭州310053 [3]杭州市第一人民医院普外科,杭州310006

出　　处：《中药新药与临床药理》2009年第6期539-542,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：杭州市科技计划项目(2005224)

摘　　要：目的研究黄芪注射液对阻塞性黄疸(OJ)大鼠小肠黏膜的保护作用及其可能的作用机制。方法取SD大鼠120只,采用丝线双重结扎胆总管近端,并切断胆总管的方法,制备OJ大鼠模型,将成模大鼠随机分为2组,每组60只,即模型组和黄芪注射液组(按7.5mL·kg-1腹腔内注射黄芪注射液),另取健康SD大鼠60只作为假手术组。各组再按术后时间分别为7、14、21和28d,共4个时相分组,每组15只。观察和记录各组大鼠的死亡情况并计算总死亡率,采用HE染色法光镜下观察大鼠小肠黏膜的病理变化情况,并检测血清丙二醛(MDA)和超氧化物歧化酶(SOD)的含量。结果黄芪注射液组大鼠的死亡数目虽减少,但与模型组比较,无统计学差异(P>0.05)。黄芪注射液组小肠黏膜病理评分(7、14d)均明显小于模型组(P<0.05)。黄芪注射液组血清MDA含量(14d)明显小于模型组(P<0.01),但是SOD含量(各时间点)明显高于模型组(P<0.05)。结论黄芪注射液可通过提高OJ大鼠血清SOD含量、减少MDA的含量,进而减轻小肠黏膜病理损害,发挥对OJ大鼠小肠黏膜的保护作用。Objective To investigate the protective effect and mechanism of Radix Astragali injection （RAI） on the intestinal mucosa of rats with obstructive jaundice （OJ）. Methods One hundred and eighty SD rats were randomly divided into sham - operation group, model control group and RAI group （gastric garage of RAI 7.5 mL · kg^- 1）, 60 rats in each group. On the 7th day, 14th day, 21st day and 28th day after operation, the mortality rates of rats, pathological changes of intestinal mucosa as well as serum MDA and SOD contents were observed, respectively. Results Compared to the model control group, the number of dead OJ rats in RAI group decreased but showed no statistical difference （ P 〉 0.05）. The pathological severity score （on 7th day and 14th day） of the intestinal mueosa and serum MDA content （on the 14th d） of RAI group were significantly lower than those in the model control group （ P 〈 0.05）. The serum SOD content （on all time points） of RAI group was significantly higher than those in the model control group （ P 〈0.05）. Conclusion Radix Astragali injection exerts protective effects on the intestinal mucosa of OJ rats through increasing SOD content, reducing MDA content and relieving the pathological changes of intestinal mucosa.

关 键 词：黄芪注射液 阻塞性黄疸 小肠黏膜 保护作用 大鼠 

分 类 号：R285.5[医药卫生—中药学]