微滤-超滤法精制苦参水煎液的工艺研究  

Research on Refining of Radix Sophorae Flavescentis Decoction by Microfiltration-Ultrafiltration Technology

作　　者：高红宁[1] 金万勤[2] 郭立玮[1] 

机构地区：[1]南京中医药大学药学院,南京210029 [2]南京工业大学,南京210009

出　　处：《中药新药与临床药理》2009年第6期571-573,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(30171161);国家中医药管理局中医药科学技术研究专项(02-03ZP33)

摘　　要：目的研究微滤与超滤联用技术精制苦参水煎液的工艺,并与醇沉法作对比。方法采用HPLC法测定氧化苦参碱的含量,紫外分光光度法测定苦参总黄酮的含量,通过选择适宜浓度的NaOH溶液作为清洗剂清洗膜。结果微滤与超滤联用技术处理的苦参水煎液的除杂率及有效成分得率均优于醇沉法,且微滤-超滤法常温下操作,周期短,膜的清洗方便。结论微滤与超滤法联用技术可替代传统的醇沉法精制苦参水煎液。Objective To study the refining of Radix Sophorae Flavescentis （RSF） decoction by microfiltration combined with ultrafiltration method, and to compare the refining effect with that refined by alcohol sedimentation method. Methods HPLC was applied to analyze the content of oxymatfine, uhroviolet （UV） spectrophotometry was used to determine the content of total flavonoids in RSF decoction, and suitable concentration of NaOH solution for the detergent were chosen in order to wash the membrane. Results The refining effect is better and the recovery ratio of efficient components is higher in RSF decoction refined by microfiltration combined with ultrafiltration method than in those refined by alcohol sedimentation method. Microfihration combined with uhrafiltration method is of simple operation and short period, and ultrafihration membranes can be easily washed. Conclusion The mierofiltration combined with ultrafiltration method can be used as the alternative for the traditional alcohol sedimentation method in refining of RSF decoction.

关 键 词：微滤 超滤 醇沉 苦参水煎液 

分 类 号：R284[医药卫生—中药学] TQ460.6[医药卫生—中医学]