关黄柏提取纯化过程中生物碱转移率的考察  

Study on the Transfer Rate of Alkaloids in Extraction and Purification Process of Cortex Phellodendri Amurensis

作　　者：陈国留[1] 刘东辉[1] 张琼丹[1] 黄海珍[1] 吴碧玉[1] 魏刚[1] 

机构地区：[1]广州中医药大学,广东广州510006

出　　处：《中药新药与临床药理》2009年第6期573-576,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金："十一五"国家科技支撑计划项目(2008BAI53B074)

摘　　要：目的对关黄柏提取纯化过程中生物碱的转移率进行考察,以确定最佳精制工艺。方法采用AB-8型大孔树脂对关黄柏生物碱进行纯化,以盐酸小檗碱和盐酸巴马汀的含量为指标,采用HPLC对醇提液、上柱液及洗脱液进行含量测定。结果最佳工艺为8倍量75%的乙醇回流提取3次,每次1h,转移率可达83.75%;上柱液(调pH=8并离心)浓度为0.1g/mL的转移率达到80%左右;上柱液浓度为0.1g/mL时洗脱液中生物碱的转移率达到75.14%。结论经过优化的工艺稳定可行,用AB-8型大孔树脂纯化关黄柏中生物碱效果良好。Objective To investigate the transfer rate of alkaloids in extraction and purification process of Cortex Phellodendri Amurensis （CPA） and to ascertain the optimal purification technology. Methods We purified the alkaloids from Cortex Phellodendri Amurensis by AB - 8 macroporous resin. With the contents of berberine hydrochloride and palmatine hydrochloride as the reference indexes, content determination of alcohol extract, sample solution and eluent from AB - 8 macroporous resin were determined by HPLC. Results The optimal technology was as follows： using 8 - fold 75 % （V/V） ethanol as extract, extracting for 3 times and lasting one hour for each time. The transfer rate of alkaloids was 83.75 % under the above condition. The transfer rate of alkaloids was about 80 % when the concentration of sample solution（pH value being 8 and centrifugation） was 0. 1 g/mL, and the transfer rate of alkaloids in eluent was 75.14 %. Conclusion The optimum technology is stable and feasible, and the purification effect of alkaloids from Cortex Phellodendri Amurensis by AB - 8 macroporus resin is good.

关 键 词：关黄柏 盐酸小檗碱 盐酸巴马汀 AB-8型大孔树脂 转移率 

分 类 号：R284[医药卫生—中药学] TQ460.6[医药卫生—中医学]