诱导大鼠产生脑缺血耐受的时间剂量探讨  

Exploration of Ischemic Preconditioning Time for Inducing Rat Brain Ischemia Tolerance

作　　者：方晓艳[1,2] 白明[1] 李建平[1] 孙建宁[2,1] 苗明三[1] 

机构地区：[1]河南中医学院,郑州450008 [2]北京中医药大学,北京100029

出　　处：《中药新药与临床药理》2009年第6期585-587,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：河南省重大公益性科研计划[豫财办教(2007)264号]

摘　　要：目的探讨诱导大鼠脑缺血耐受产生的缺血预处理时间剂量。方法采用前脑-局灶脑缺血耐受模型,短暂性阻断双侧颈总动脉造成前脑缺血预处理,72h后行最后一次局灶性脑缺血2h(中动脉栓塞,MCAO),脑缺血预处理时间剂量分别为10min、20min,通过测定脑梗死面积百分率,探讨诱导大鼠脑缺血耐受产生的最佳缺血预处理时间剂量。结果10min和20min短暂的脑缺血预处理均可以保护大脑抵御下次严重的缺血,使缺血梗死面积显著缩小;10min缺血预处理时间组和20min缺血预处理时间组相比没有显著性差异,但10min缺血预处理时间组脑梗死面积百分率小于20min缺血预处理时间组。结论10min缺血预处理时间是较好的诱导脑缺血耐受产生的时间剂量。Objective To investigate the ischemic preconditioning time for inducing rat brain ischemia tolerance. Methods Procerebrum ischemie preconditioning injury was caused by occlusion of two- side common carotid artery for 10 minutes or 20 minutes, and subsequent cerebral ischemic injury was caused by occlusion of middle cerebral artery for 2 hours after 72 hours of reperfusion. The percentage of brain infarct size was calculated for the investigation of the proper ischemic preconditioning time for rat brain isehemia tolerance. Results Ischemic preconditionaing for 10 minutes and 20 minutes can reduce the percentage of brain infarct size significantly. The difference of the percentage of brain infarct size between 10 - minute preconditioning group and 20 - minute preconditioning group is insignificant, but the mean percentage of brain infarct size in 10 - minute preconditioning group is less than that in 20 - minute preconditioning group. Conclusion Ten minutes are the suitable time of ischemic preconditioning for rat brain isehemia tolerance.

关 键 词：脑缺血 缺血预处理 时间剂量 梗死面积 

分 类 号：R285.5[医药卫生—中药学]