Isolation and identification of Rhodosporidium diobovatum DS-0205 from deep-sea sediment of eastern Pacific Ocean  

作　　者：曾乐平 黄菊芳 邱冠周 初凤友 陈旦 童建斌 罗学港 

机构地区：[1]Xiangya School of Medicine,Central South University [2]Key Laboratory of Submarine Geosciences of State Oceanic Administration [3]School of Resources Processing and Bioengineering,Central South University

出　　处：《Journal of Central South University》2009年第6期942-947,共6页中南大学学报（英文版）

基　　金：Projects(40776035, 40376036) supported by the National Natural Science Foundation of China;Project(DY105-02-04-05) supported by the China Ocean Mineral Resources Research and Development Association;Project(07MX27) supported by the Mittal Student Foundation;Project (2340-74236000003) supported by the Post-graduate Foundation of Hunan Province, China;Project (200805330053) supported by the Doctoral Foundation of Ministry of Education of China

摘　　要：A facultative heterotrophic strain （DS-0205） was isolated from a deep-sea sediment sample collected at a depth of 5.2 km in the eastern Pacific Ocean, China. Strain DS-0205 is motile helmet-like single cell or pairs, forming hemisphere with the center sunken of variable size. It has a widespread carbon source and nitrogen source, including agarose, citric acid, salicin, D-glucitol nitrate, sodium nitrite and cthylamine, tt can grow in the following environment： temperature 4-37℃, pH 2.0-12.0, tolerance to NaCl≤15%. Two phylogenetic trees, one based on the ITS and 5.8S rRNA sequences and the other based on the 18S rRNA sequences, unite strain DS-0205（=JCM 0205） to the type strain of Rhodosporidium diobovatum JCM 3787 through a considerable evolutionary distance. These results suggest that strain DS-0205 is a new strain of the Rhodosporidium diobovatum.A facultative heterotrophic strain (DS-0205) was isolated from a deep-sea sediment sample collected at a depth of 5.2 km in the eastern Pacific Ocean,China. Strain DS-0205 is motile helmet-like single cell or pairs,forming hemisphere with the center sunken of variable size. It has a widespread carbon source and nitrogen source,including agarose,citric acid,salicin,D-glucitol nitrate,sodium nitrite and ethylamine. It can grow in the following environment:temperature 4-37 ℃,pH 2.0-12.0,tolerance to NaCl≤15%. Two phylogenetic trees,one based on the ITS and 5.8S rRNA sequences and the other based on the 18S rRNA sequences,unite strain DS-0205(=JCM 0205) to the type strain of Rhodosporidium diobovatum JCM 3787 through a considerable evolutionary distance. These results suggest that strain DS-0205 is a new strain of the Rhodosporidium diobovatum.

关 键 词：deep-sea sediment ITS and 5.8S rRNA sequences 18S rRNA ISOLATION IDENTIFICATION 

分 类 号：P736.21[天文地球—海洋地质]