SD大鼠层粘蛋白受体基因的克隆与序列分析  

Cloning and sequence analysis of laminin receptor gene of SD rat

作　　者：宁章勇[1] 李建军[2] 罗敏意[1] 程艳芬[1] 潘家强[1] 于博[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科技学院动物医学系,广东佛山528231

出　　处：《中国兽医杂志》2009年第11期3-5,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(30700603);广东省自然科学基金(7300744);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)

摘　　要：以大鼠大脑皮质神经元的总RNA为模板,运用RT-PCR方法扩增了SD大鼠层粘蛋白受体基因,并将其克隆到pMD18-T Simple载体中进行测序,运用分子生物学软件对获得序列进行了分析。结果表明所克隆的SD大鼠层粘蛋白受体基因的ORF片段包含了朊蛋白基因的完整编码区,共888个核苷酸,该基因内无内含子,编码295个氨基酸的前体蛋白,推测其相对分子质量约32.6 kD。与已报道的绵羊、家鼠、猪、牛、人相应序列作比较,发现其核苷酸序列和氨基酸序列具有较高的同源性。氨基酸序列分析表明,SD大鼠层粘蛋白受体的氨基酸点突变位点为V176M,G243E。研究结果为探讨层粘连蛋白受体基因的功能及其在疾病发生、发展过程中的作用提供了基础数据。Laminin receptor gene of SD rat was amplified by RT-PCR with the total RNA extracted from the cortical neurons. The gene was sequenced after cloned into the vector pMD18-T Simple and analyzed with molecular biological software. The results showed that the cloned opening reading frame （ORF） of laminin receptor gene of SD rat consists of 888 nucleotides and contains the entire laminin receptor gene coding sequence that has no intron. The encoded precursor protein is 295 amino acids and molecular weight （MW） is 32.6 KD. The gene shared higher homology with the sheep, mouse, pig, cowand human genes reported in GenBank. V176M and G243E amino acid mutations were found in laminin receptor of SD rat. The results provided the basic data for investigating into the role of laminin receptor in the onset and progession of the disease.

关 键 词：SD大鼠 层粘蛋白受体 序列分析 

分 类 号：Q785[生物学—分子生物学]