pSVPoMcat微基因在雪旺氏细胞中的表达  

The expression of pSVPoMcat gene in Schwann Cells

作　　者：陈礼刚 高立达[2] 胡威夷[1] 毛伯镛 陈俊杰[3] 赖立辉[3] 曾凡俊[1] 

机构地区：[1]成都军区总医院神经外科,610083 [2]华西医大附属一医院神经外科 [3]华西医大基础医学院重组DNA研究室

出　　处：《中华实验外科杂志》1998年第6期566-568,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨pSVPoMcat微基因表达产物对体外培养雪旺氏细胞(SC)的影响。方法在构建Po-5′-flanking介导的21.5KDhMBP微基因的基础上,采用Lipofectanine将pSVPoMcat转染至体外纯化培养的SC中,通过CAT-ELISA、hMBP-ELISA、ABC抗hMBP的免疫组化以及MTT法等多种方法,了解该微基因对SC的作用。结果转有pSVPoMcat基因的SC其细胞蛋白含量、CAT酶表达量、MBP的表达量及生长增殖能力明显高于未经转染的SC。结论 Po-5′-flanking具有指导MBPcDNA在SC中特异表达的功能;pSVPoMcat微基因表达对SC的增殖有明显的促进作用。Objective To explore the effect of pSVPoMeat gene on cultured Schwann cells (SC) in vivo. Meth-ods The plasmid pSVPoMcat was constructed as a vector containing the gene of 21. 5 ku human myelin basic protein (hMBP) mediated by Po-5~1-flanking and transferred into cultured purified SCs by lipofec-tamine. CAT-ELISA, hMBP-ELISA, ABC anti-hNBP immunoeytochemistry, and MTT assay were used to confirm and determine the expression of hMBP cDNA in vivo. Results In the SC pSVPoMcat geneti-cally modified, the protein content of cell lysate, the amount of CAT enzyme, and the amount of MBP ex-pression were higher than those of the nontransfected SCs. Conclusion Po-5~1-flanking had the function of guiding hMBPcDNA speefric expression in SCs. The expression of pSVPoMcat would significantly pro-mote the growth and proliferation of SCs.

关 键 词：髓鞘碱性蛋白 基因 雪旺氏细胞 体外细胞培养 

分 类 号：Q343.6[生物学—遗传学] R651[医药卫生—外科学]