鸭脂蛋白脂酶基因内含子5多态性及其与肉质和脂肪性状的关联分析  

Polymorphism of Intron 5 of Lipoprotein Lipase Gene and Its Relationship with Meat Quality and Fat Traits in Ducks

作　　者：张依裕[1,2] 徐琪[1] 段修军 张海波[1] 孙国波 赵文明[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]贵州大学动物科学学院,贵州贵阳550025 [3]江苏省畜牧兽医职业技术学院,江苏泰州225300

出　　处：《中国家禽》2009年第22期25-28,共4页China Poultry

基　　金：国家科技部支撑计划重大专项(2006BDA01A09);国家科技部支撑计划重点项目(2006BDA14B06);现代农业产业技术体系建设专项资金(nycytx-45-04)

摘　　要：根据鸡脂蛋白脂酶(LPL)基因序列设计引物对樱桃谷鸭和苏牧麻鸭LPL基因内含子5进行PCR-SSCP检测,并分析其多态对肉质和脂肪性状的遗传效应。结果表明,检测到3种基因型:AA、AB和BB,1个双碱基突变:243～244bp(TA→AT),2个SNP:G265A和G269A;该座位在2个群体中均为中度多态,并偏离Hardy-Weinberg平衡(P<0.05),基因型分布差异显著(P<0.05);在樱桃谷鸭群体中,AB型个体的pH值显著高于AA型,失水率显著高于BB型(P<0.05);苏牧麻鸭中AB型个体的总胆固醇显著高于AA和BB型个体(P<0.05)。The fragment of intron 5 of lipoprotein lipase （LPL） gene was amplified on the genome DNA of two ducks populations （Cherry Valley duck and Sumu duck）with the primer designed from chicken LPL sequence. The fragment polymorphism was detected by PCR-SSCP method,and its relationship with meat quality and fat traits of two duck populations were analysed.The results showed that a two-base mutation and two SNPs were detected （TA243-244AT,G265A and G269A）,whieh resulted in three genetypes （AA,AB and BB）. The loci presented moderate polymorphic and deviated from the Hardy-Weinberg equilibrium in two duck populations （P〈0.05）. Chi-Square test indicated that genotypes distribution of the two duck populations were significant （P〈0.05）. The pH value of AB genotype of Cherry Valley duck population was significantly higher than AA genotype （P〈0.05）,and percentage of loss water was also significantly higher than BB genotype （P〈0.05）. Total cholesterol of AB genotype were significantly higher than AA and BB genotype in Suma duck population.

关 键 词：家鸭 LPL基因 PCR—SSCP 性状 关联性 

分 类 号：S852.239.1[农业科学—基础兽医学] S831.2[农业科学—兽医学]