产AmpC酶肺炎克雷伯菌的耐药性及基因型研究  

A study on resistance and genotypes of AmpC beta-lactamase producing Klebsiella pneumoniae in Anhui province

作　　者：朱玉林[1] 高帆[1] 张晓妮[1] 程君[1] 殷俊[1] 李家斌[1,2] 叶英[1,2] 

机构地区：[1]安徽医科大学第一附属医院感染科,合肥230022 [2]安徽省细菌耐药监测中心,合肥230022

出　　处：《临床检验杂志》2009年第6期453-455,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(30772286);安徽省自然科学基金(070413110)

摘　　要：目的了解安徽地区产AmpC酶肺炎克雷伯菌的基因型特征及耐药性。方法对180株肺炎克雷伯菌进行头孢西丁纸片法初筛,三维实验筛选高产AmpC酶菌,PCR扩增、测序和BLAST比对分析以确定AmpC基因型,琼脂稀释法检测耐药性。结果产AmpC酶菌株有21株(11.67%),其中DHA型17株,EBC型4株。3株EBC型为新基因(gene bank登录号为FJ237366,FJ237367,FJ237368);药敏显示产酶株除对亚胺培南与美罗培南敏感外,对其他抗菌药物均有不同程度的耐药。结论安徽地区产AmpC酶的肺炎克雷伯菌以DHA型为主,同时存在EBC型突变株。高产AmpC酶菌的感染推荐使用碳青霉烯类抗生素治疗。Objective To identify the plasmid-mediated AmpC gene and to investigate its prevalence in Klebsiella pneumoniae strains isolated in Anhui Province.Methods The AmpC-producing isolates were chosen by cefoxitin and identified by the three-dimensional test.The plasmid-mediated AmpCβ-lactamases were detected by multiplex PCR.The PCR products were directly sequenced and analyzed. M-H agar dilution method was used to determine MIC of 17 antimicrobial agents against the AmpC positive isolates.Results Of the 180 strains,21(11.67%) proved to be plasmid-mediated highly productive AmpC by DNA sequence test.Blast results indicated that the positive AmpC group was composed of 17 strains which carried DHA type and 4 strains which carried EBC type.DNA sequence analysis revealed three novel AmpC genotypes(GenBank accession:FJ237366,FJ237367,and FJ237368).All AmpC positive isolates exhibited high resistance to the third or fourth generation cephalosporins,aminoglycosides,and quinolones.But all of them were susceptible to imipenem and meropenem.Conclusions The plasmid-mediated AmpCβ-lactamases were found in Klebsiella pneumoniae strains isolated in Anhui Province and DHA type was dominant.Moreover,three novel AmpC genotypes were identified.The carbapenems are recommended to treat the AmpC-producing isolates.

关 键 词：AMPC酶 肺炎克雷伯菌 耐药性 基因型 

分 类 号：R446.5[医药卫生—诊断学]