SP-1在TNFα诱导内皮细胞HO-1基因表达中的作用  

Roles of SP-1 on TNFα induced expression of HO-1 gene in endothelial cells

作　　者：林宇[1] 孙超[1] 杨宏艳[1] 苏富琴[1] 李清漪[1] 徐启华[1] 林岩[1] 

机构地区：[1]齐齐哈尔医学院药理教研室,161005

出　　处：《齐齐哈尔医学院学报》2009年第21期2618-2619,共2页Journal of Qiqihar Medical University

基　　金：黑龙江省自然科学基金资助(No:ZA2006-05)

摘　　要：目的探讨转录因子SP-1在肿瘤坏死因子TNFα诱导血管内皮细胞血红素氧化酶HO-1基因表达中的作用。方法用ELISA法检测HO-1的蛋白表达,用Westernblot法检测SP-1的蛋白水平。结果TNFα(200U/ml)处理24h后,HUVEC胞内HO-1和AP-1蛋白水平均明显升高;NAC(20mmol/L)预处理可明显抑制胞浆内AP-1及HO-1的蛋白含量(P<0.05)。结论SP-1部分介导了TNFα对HO-1基因表达的诱导作用。Objective To investigate the roles of specificity protein-1（SP-1）on tumor necrosis factor-α（TNFα）-induced expression of HO-1 in endothelial cells.Methods The expressions of the HO-1 and SP-1 at the protein were measured by ELISA and western blot.Results TNFα（200U/ml）treatment caused an increase in the protein contents of the HO-1 and SP-1,which were markedly reduced by pretreatment with NAC（20mmol/L）.Conclusions SP-1 may play a major role in the TNFα-induced upregulation of HO-1 gene.

关 键 词：肿瘤坏死因子-Α 血管内皮细胞 血红素氧化酶-1 SP-1 

分 类 号：R543[医药卫生—心血管疾病] R654.4[医药卫生—内科学]