GPI锚定蛋白抗体CD55、CD59及RET在贫血诊断中的意义探讨  

Investigation of anchoring protein antibodies CD55/CD59 and RET on the diagnostic significance of anemic patients

作　　者：钟琼[1] 马丽[2] 

机构地区：[1]广东医学院附属医院检验科,广东湛江524023 [2]广东医学院检验室,广东东莞523808

出　　处：《中国实验诊断学》2009年第11期1566-1569,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的检测几类贫血患者外周血中红细胞和中性粒细胞膜上糖化磷脂酰肌醇(GPI)连接的补体调节蛋白CD55和CD59表达情况,综合分析各类贫血中网织红细胞(RET)的变化特点,探讨他们在贫血中的诊断价值。方法荧光标记的CD55、CD59单克隆抗体,用流式细胞技术及亚甲蓝活体染色检测,30例正常人、25例缺铁性贫血(IDA)、26例巨幼细胞性贫血(MA)2、7例自身免疫溶血性贫血(AHA),27例再生障碍性贫血、21例再生障碍性贫血-阵发性睡眠性血红蛋白尿症(AA-PNH)3、0例阵发性睡眠性血红蛋白尿症(PNH)患者外周血中CD55-和CD59-红细胞和中性粒细胞的百分率及RET值。结果正常人CD55-、CD59-红细胞和中性粒细胞的百分率均<5%,44%的IDA患者CD55-、CD59-红细胞百分率在5%-20%之间,中性粒细胞的百分率<5%,MA及AHA患者CD55-、CD59-红细胞和中性粒细胞百分率均<5%。33%AA患者CD55-、CD59-细胞百分率在5%-15%之间。PNH及AA-PNH患者CD55-、CD59-细胞百分率均>11%。与正常对照组比较,各类贫血间RET值差异显著(P<0.01)。结论IDA、AA、AA-PNH、及PNH外周血中CD55-、CD59-百分率较正常对照组有不同程度增加,PNH最为明显,IDA变化最小。联合RET检查有助于各类贫血的诊断、鉴别诊断及愈后判断。Objective By detecting the expression of membrane associated complement regulatory proteins （CRP）, CD55 and CD59 on the peripheral blood cerythrocytes and neutrophils in several kinds of anemia patients, and analysing the percentages of reticuloc, yte to investigate the diagnostic value of them in anemic patients. Methods the percentages of CD55^-/CD59^- and reticulocyte （Ret % ）of 30 normal subjects, 25 cases of IDA, 26 cases of MA, 27 cases of AliA, 27 cases of AA, 21 cases AA-PNH and 30 cases of PNH patients were detected by luorescence-activated cell sorting （FACS） with PE and F1TC fluorescence labeled anti-CD55 and anti- CD59 monoclonal antibodies, and by methylene blue intravital staining technology. Results The percentage of CD55^- and CD59^- cells in 30 normal subjects were less than 5%. The percentages of CD55^- and CD59^- on erythrocytes were in the range from 5% to 20 %, on neutrophils were less than 5 % in 44% of the patients with microcytic hypochromic anemia. The percentages of CD55^- and CD59^- on erythrocytes and neutrophils were less than 5 % in patients with maerocytic anemia and autoimmune hemolytic anemia. The percentages of CD55^- and CD59^- on erythrocytes and neutrophils were in the range of 5% to 15% in about 33% patients with aplas- tic anemia, but the percentages in the patients with PNH and AA-PNH were more than 11%. compare to normal control,the percent- ages of RET in several kind of anaemia were statistics significant （ P 〈 0.01 ）. Conclusion The percentage of CD55^-/CD59^- in patients with IDA, AA, AA-PNH increased distinctly, especially in the patients with PNH obvious. It would be helpful for the diagnosis. antidiastole and prognosis when combination of CD55/CD59and BET.

关 键 词：贫血 CD55 CD59 网织红细胞 

分 类 号：R556.64[医药卫生—血液循环系统疾病]