Layer-By-Layer技术构建化学传感器酶电极检测水体中有机磷的研究  

Study on Chemical Sensor Fabrication by Layer-by-layer Technique for Organophosphate Determination in Water

作　　者：李其云 冷鹏 

机构地区：[1]济南第一中学,山东济南250100 [2]山东省环境保护学校,山东济南250014

出　　处：《中国环境管理干部学院学报》2009年第4期73-76,87,共5页Journal of Environmental Management College of China

摘　　要：基于层层累积自组装法将胆碱氧化酶和胆碱酯酶逐层固定在电极表面，制备了电流型水质有机磷检测化学传感器。讨论了自组装膜层数、pH值、温度对传感器电流响应的影响。制备的化学传感器对有机磷在浓度10^-9～10^-5mol／L呈良好的线性响应，检出限为1×10^-10mol／L。传感器的稳定性好，30天时的响应值仍保持90％。An amperemetric chemical sensor is fabricated by immobilization of choline oxidase and acetyl cholinesterase on the surface of electrode with layer-by-layer self-assembled monolayer technique for detection of organophosphorus pesticide in water. This paper studies the effects of layers of self-assembled monolayer, pH, temperature on the response current. This chemical sensor exhibites a good storage stability and remains 90% activity over 30 days when the linear range of the prepared chemical sensor was 10^-9-10^-5 mol/L and the detection limit was 1.0×10^-10 mol/L.

关 键 词：有机磷 化学传感器 层层累积自组装膜技术 胆碱氧化酶 胆碱酯酶 

分 类 号：X832[环境科学与工程—环境工程]